1. Recombinant Proteins
  2. Cytokines and Growth Factors CD Antigens Receptor Proteins
  3. Interleukin & Receptors Monocyte CD Proteins Cytokine Receptors
  4. IL-1R CD121b/IL-1 Receptor 2
  5. IL-1 Receptor 2 (IL-1R2)
  6. IL-1R2 Protein, Mouse (HEK293, His)

IL-1R2 Protein, Mouse (HEK293, His)

Cat. No.: HY-P72568
COA Handling Instructions

IL-1R2 is a competitive endogenous inhibitor of IL-1 signaling. IL-1R2 serves as a decoy receptor and competes with IL-1R1 for IL-1, it can also form a complex with IL-1RAP once it binds IL-1. IL-1R2 is implicated in various IL-1-mediated inflammatory diseases. IL-1R2 Protein, Mouse (HEK293, His) is a recombinant mouse IL-1R2 protein with a His tag at the C-terminus and is expressed in HEK293 cells. It consists of 342 amino acids (F14-E355).

For research use only. We do not sell to patients.

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Description

IL-1R2 is a competitive endogenous inhibitor of IL-1 signaling. IL-1R2 serves as a decoy receptor and competes with IL-1R1 for IL-1, it can also form a complex with IL-1RAP once it binds IL-1. IL-1R2 is implicated in various IL-1-mediated inflammatory diseases[1]. IL-1R2 Protein, Mouse (HEK293, His) is a recombinant mouse IL-1R2 protein with a His tag at the C-terminus and is expressed in HEK293 cells. It consists of 342 amino acids (F14-E355).

Background

IL-1R2 is the non-signaling type 2 interleukin-1 receptor and is expressed in DC, CD4+ T cells, T regulatory cells, monocytes, neutrophils and different macrophagic cells in mouse. The mouse IL-1R2 can be cleaved into two forms: membrane form (14-410 a.a) and soluble form (14-? a.a).
The sequence of amino acids in IL-1R2 from mouse shows low similarity (about 60%) with human IL-1R2, but has been only slightly altered with rat IL-1R2 (90 % similarity).
IL-1R2 contains truncated cytoplasmic domain and lacks Toll-IL-1 receptor (TIR) region, making it incapable of transmembrane signaling. IL-1R2 serves as an endogenous inhibitor of IL-1 signaling. Functional IL-1 signaling requires IL-1R1 and IL-1-dependent recruitment of IL-1RAP. IL-1R2 serves as a decoy receptor and can compete with IL-1R1 for IL-1. IL-1R2 can also form a complex with IL-1RAP once it binds IL-1, preventing IL-1RAP from heterodimerizing with IL-1R1. Through these 2 ways, IL-1R2 blocks IL-1 signaling. Additionally, soluble IL-1R2 recruits soluble IL-1RAP with high affinity without impacting affinity for IL-1RA.
IL-1R2 inhibits IL-1 signaling and has been implicated in various IL-1-mediated inflammatory diseases like arthritis, diabetes, gout and so on[1][2][3].

In Vitro

IL-1R2 (24 h) specifically inhibits IL-1β-induced urokinase-type plasminogen activator responses in HUVECs[4].

In Vivo

IL-1R2 knockout mice exacerbates cardiac damage in myocardial ischemia reperfusion (I/R) injury and knockout of IL-1R2 induces cytokines expression in the heart after I/R injury[3].
AAV9-IL-1R2 (40 µL; myocardial injection, once) protects heart against IL-17A-induced cardiomyocytes apoptosis through suppressing IL-17RA expression in mice[3].

Species

Mouse

Source

HEK293

Tag

C-6*His

Accession

P27931 (F14-E355)

Gene ID

16178  [NCBI]

Molecular Construction
N-term
IL-1R2 (F14-E355)
Accession # P27931
6*His
C-term
Synonyms
Interleukin-1 receptor type 2; IL-1R-2; CDw121b; IL-1R-beta; CD121b; mIL-1R2; sIL-1R2
AA Sequence

FTTPTVVHTGKVSESPITSEKPTVHGDNCQFRGREFKSELRLEGEPVVLRCPLAPHSDISSSSHSFLTWSKLDSSQLIPRDEPRMWVKGNILWILPAVQQDSGTYICTFRNASHCEQMSVELKVFKNTEASLPHVSYLQISALSTTGLLVCPDLKEFISSNADGKIQWYKGAILLDKGNKEFLSAGDPTRLLISNTSMDDAGYYRCVMTFTYNGQEYNITRNIELRVKGTTTEPIPVIISPLETIPASLGSRLIVPCKVFLGTGTSSNTIVWWLANSTFISAAYPRGRVTEGLHHQYSENDENYVEVSLIFDPVTREDLHTDFKCVASNPRSSQSLHTTVKE

Molecular Weight

45-60 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
IL-1R2 Protein, Mouse (HEK293, His)
Cat. No.:
HY-P72568
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