IL-1R2 Protein, Mouse (HEK293, hFc)
IL-1R2 is a competitive endogenous inhibitor of IL-1 signaling. IL-1R2 serves as a decoy receptor and competes with IL-1R1 for IL-1, it can also form a complex with IL-1RAP once it binds IL-1. IL-1R2 is implicated in various IL-1-mediated inflammatory diseases. IL-1R2 Protein, Mouse (HEK293, hFc) is a recombinant mouse IL-1R2 protein with an hFc tag at the C-terminus and is expressed in HEK293 cells. It consists of 355 amino acids (M1-E355).
- Species: Mouse
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
IL-1R2 is a competitive endogenous inhibitor of IL-1 signaling. IL-1R2 serves as a decoy receptor and competes with IL-1R1 for IL-1, it can also form a complex with IL-1RAP once it binds IL-1. IL-1R2 is implicated in various IL-1-mediated inflammatory diseases[1]. IL-1R2 Protein, Mouse (HEK293, hFc) is a recombinant mouse IL-1R2 protein with an hFc tag at the C-terminus and is expressed in HEK293 cells. It consists of 355 amino acids (M1-E355).
IL-1R2 is the non-signaling type 2 interleukin-1 receptor and is expressed in DC, CD4+ T cells, T regulatory cells, monocytes, neutrophils and different macrophagic cells in mouse. The mouse IL-1R2 can be cleaved into two forms: membrane form (14-410 a.a) and soluble form (14-? a.a).
The sequence of amino acids in IL-1R2 from mouse shows low similarity (about 60%) with human IL-1R2, but has been only slightly altered with rat IL-1R2 (90 % similarity).
IL-1R2 contains truncated cytoplasmic domain and lacks Toll-IL-1 receptor (TIR) region, making it incapable of transmembrane signaling. IL-1R2 serves as an endogenous inhibitor of IL-1 signaling. Functional IL-1 signaling requires IL-1R1 and IL-1-dependent recruitment of IL-1RAP. IL-1R2 serves as a decoy receptor and can compete with IL-1R1 for IL-1. IL-1R2 can also form a complex with IL-1RAP once it binds IL-1, preventing IL-1RAP from heterodimerizing with IL-1R1. Through these 2 ways, IL-1R2 blocks IL-1 signaling. Additionally, soluble IL-1R2 recruits soluble IL-1RAP with high affinity without impacting affinity for IL-1RA.
IL-1R2 inhibits IL-1 signaling and has been implicated in various IL-1-mediated inflammatory diseases like arthritis, diabetes, gout and so on[1][2][3].
IL-1R2 (24 h) specifically inhibits IL-1β-induced urokinase-type plasminogen activator responses in HUVECs[4].
IL-1R2 knockout mice exacerbates cardiac damage in myocardial ischemia reperfusion (I/R) injury and knockout of IL-1R2 induces cytokines expression in the heart after I/R injury[3].
AAV9-IL-1R2 (4 µL; myocardial injection, once) protects heart against IL-17A-induced cardiomyocytes apoptosis through suppressing IL-17RA expression in mice[3].
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-hFc
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Accession
P27931 (F14-E355)
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Molecular Construction
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N-term
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IL-1R2 (F14-E355)
Accession # P27931 -
hFc
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C-term
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Protein Length
Extracellular Domain
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Synonyms
Interleukin-1 receptor type 2; IL-1R-2; CDw121b; IL-1R-beta; CD121b; mIL-1R2; sIL-1R2
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AA Sequence
FTTPTVVHTGKVSESPITSEKPTVHGDNCQFRGREFKSELRLEGEPVVLRCPLAPHSDISSSSHSFLTWSKLDSSQLIPRDEPRMWVKGNILWILPAVQQDSGTYICTFRNASHCEQMSVELKVFKNTEASLPHVSYLQISALSTTGLLVCPDLKEFISSNADGKIQWYKGAILLDKGNKEFLSAGDPTRLLISNTSMDDAGYYRCVMTFTYNGQEYNITRNIELRVKGTTTEPIPVIISPLETIPASLGSRLIVPCKVFLGTGTSSNTIVWWLANSTFISAAYPRGRVTEGLHHQYSENDENYVEVSLIFDPVTREDLHTDFKCVASNPRSSQSLHTTVKE
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Predicted Molecular Mass
65.1 kDa
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Molecular Weight
Approximately 91 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
N/A.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
References
[1]. PetersVA,etal.IL-1receptor2(IL-1R2)anditsroleinimmuneregulation.BrainBehavImmun.2013Aug;32:1-8. [Content Brief]
[2]. Molgora M, et al. Tuning inflammation and immunity by the negative regulators IL-1R2 and IL-1R8. Immunol Rev. 2018 Jan;281(1):233-247. [Content Brief]
[3]. Lin J, et al. Cardiomyocyte IL-1R2 protects heart from ischemia/reperfusion injury by attenuating IL-17RA-mediated cardiomyocyte apoptosis. Cell Death Dis. 2022 Jan 27;13(1):90. [Content Brief]
[4]. Simeoni E, et al. Gene transfer of a soluble IL-1 type 2 receptor-Ig fusion protein improves cardiac allograft survival in rats. Eur J Cardiothorac Surg. 2007 Feb;31(2):222-8. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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