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  4. IL-2 Receptor CD25/IL-2R alpha
  5. IL-2R alpha
  6. IL-2R alpha/CD25 Protein, Human (Biotinylated, HEK293, His-Avi)

IL-2R alpha/CD25 Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P78092
Handling Instructions

IL-2R alpha (CD25) is an essential component of high-affinity IL-2 receptors. IL-2R alpha enhances binding of IL-2 to its receptor complex so that regulates T cell growth and other lymphoid functions. IL-2R alpha/CD25 Protein, Human (Biotinylated, HEK293, His-Avi) is a biotinylated recombinant human IL-2R alpha protein with a His tag and an Avi tag at the C-terminus and is expressed in HEK293 cells. It consists of 192 amino acids (E22-C213).

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
20 μg $245 In-stock
Estimated Time of Arrival: December 31
50 μg $540 In-stock
Estimated Time of Arrival: December 31
100 μg $918 Get quote
250 μg   Get quote  

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

  • References

Description

IL-2R alpha (CD25) is an essential component of high-affinity IL-2 receptors. IL-2R alpha enhances binding of IL-2 to its receptor complex so that regulates T cell growth and other lymphoid functions[1][2]. IL-2R alpha/CD25 Protein, Human (Biotinylated, HEK293, His-Avi) is a biotinylated recombinant human IL-2R alpha protein with a His tag and an Avi tag at the C-terminus and is expressed in HEK293 cells. It consists of 192 amino acids (E22-C213).

Background

IL-2R alpha (CD25) is a type I membrane protein. IL-2R alpha is expressed in peripheral activated T and B cells, triple-negative thymocytes, and bone marrow pre-B cells. In high tumor regulatory T (Treg) cells, IL-2R alpha is highly expressed and is a potential target for Treg deletion. The expression of IL-2R alpha is undetectable on resting T cells[1][2][3].
The sequence of amino acids in IL-2R alpha from different species is very different (less than 85% similarity among human, rat and mouse).
IL-2R alpha is an essential component of high-affinity IL-2 receptors and has no signal-transducing activity per se. IL-2R alpha functions through enhancing binding of IL-2 to its receptor complex and acts as a positive feedback regulator. IL-2 is a principal growth factor for T lymphocytes and plays an important role in T cell immune response. IL-2R alpha transcription is regulated by three positive regulatory regions (PRRs): PRRI, PRRII and PRRIII. PRRIII is an IL-2 response element[1][2].
IL-2R alpha regulates T cell growth, augments lymphocyte activation and proliferation. IL-2R alpha is involved in preventing type 1 diabetes and cancers[1][2][4].

Biological Activity
Immobilized Biotinylated Human IL-2 R alpha His at 0.2 μg/mL (100μL/Well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody hFc with the EC50 of 30.8 ng/mL determined by ELISA.
Species

Human

Source

HEK293

Tag

C-Avi;C-His

Accession

P01589 (E22-C213)

Gene ID
Synonyms
CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR
Molecular Weight

40-50 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE. Greater than 95% as determined by HPLC.

Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References
  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
IL-2R alpha/CD25 Protein, Human (Biotinylated, HEK293, His-Avi)
Cat. No.:
HY-P78092
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