Integrin alpha 2 beta 1 Protein, Mouse (HEK293, His)

Integrin alpha-2/beta-1, a pivotal collagen receptor, centralizes platelet and cell adhesion to collagens, modulating collagen and collagenase gene expression. Beyond collagen-related functions, it contributes to force generation, extracellular matrix organization, and recognizes laminins, collagen C-propeptides, and E-cadherin. Mice with a null mutation in the alpha-2 subunit face early mortality, emphasizing its critical role in developmental processes. Structurally, forming a heterodimeric complex with beta-1, Integrin alpha-2/beta-1 interacts with HPS5 and RAB21, revealing multifaceted regulatory and signaling functions. Integrin alpha 2 beta 1 Protein, Mouse (HEK293, His) is a recombinant protein dimer complex containing mouse-derived Integrin alpha 2 beta 1 protein, expressed by HEK293, with C-His labeled tag. Integrin alpha 2 beta 1 Protein, Mouse (HEK293, His), has molecular weight of 128-138 kDa (ITGA2) & 105-115 kDa (ITGB1), respectively.

The Integrin alpha-2/beta-1 protein serves as a pivotal collagen receptor, playing a central role in platelet and cell adhesion to collagens, as well as in the modulation of collagen and collagenase gene expression. Beyond its collagen-related functions, it contributes to force generation and the organization of newly synthesized extracellular matrix. Moreover, Integrin alpha-2/beta-1 acts as a versatile receptor, recognizing laminins, collagen C-propeptides, and E-cadherin. In embryogenesis, mice with a null mutation in the alpha-2 subunit face early mortality, underscoring the critical role of Integrin alpha-2/beta-1 in developmental processes. Structurally, it forms a heterodimeric complex, with the alpha-2 subunit associating with beta-1. The protein further interacts with HPS5 and RAB21, revealing additional dimensions to its regulatory and signaling functions. Overall, Integrin alpha-2/beta-1 emerges as a key player in mediating cellular interactions with the extracellular matrix and influencing essential developmental processes.

Mouse

HEK293

C-His

Q62469 (Y27-T1129)&P09055-1 (Q21-D728)

16398

128-138 kDa(ITGA2) & 105-115 kDa(ITGB1)

Greater than 95% as determined by reducing Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.