Integrin alpha L beta 2 Protein, Human (CHO, His)
Based on 1 Customer Validation
Integrin αLβ2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4, and F11R, involved in immune phenomena including leukocyte-endothelial cell interactions, cytotoxic T cell killing, and antibody-dependent killing. It contributes to the cytotoxicity of natural killer cells and leukocyte adhesion and migration. Integrin alpha L beta 2, Human (CHO, His) is a recombinant human ntegrin alpha L beta 2 protein expressed by CHO cells with a C-His tag.
- Species: Human
- Source: CHO
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Integrin αLβ2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4, and F11R, involved in immune phenomena including leukocyte-endothelial cell interactions, cytotoxic T cell killing, and antibody-dependent killing. It contributes to the cytotoxicity of natural killer cells and leukocyte adhesion and migration. Integrin alpha L beta 2, Human (CHO, His) is a recombinant human ntegrin alpha L beta 2 protein expressed by CHO cells with a C-His tag.
Background
Integrin alpha L beta 2 (ITGAL/ITGB2) serves as a receptor for ICAM1, ICAM2, ICAM3, and ICAM4, and also interacts with the secreted form of ubiquitin-like protein ISG15 through its ITGAL subunit. Additionally, it functions as a receptor for F11R and plays a role in immune processes such as leukocyte-endothelial cell interaction, cytotoxic T-cell-mediated killing, and antibody-dependent killing by granulocytes and monocytes. ITGAL/ITGB2 contributes to natural killer cell cytotoxicity, as well as leukocyte adhesion and transmigration, involving T-cells and neutrophils. Its significance extends to the generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis. Furthermore, in association with ICAM3, ITGAL/ITGB2 contributes to apoptotic neutrophil phagocytosis by macrophages. The heterodimeric structure comprises an alpha subunit (ITGAL) and a beta subunit (ITGB2), with the alpha subunit interacting with THBD. This multifaceted functionality underscores the diverse roles of Integrin alpha L beta 2 in various immune and cellular processes.
Verified Bioactivity
Measured by its binding ability in a functional ELISA.Immobilized Human ITGAL&ITGB2 Heterodimer Protein, His Tag&Tag Free at 10 μg/mL (100 μL/well) can bind Human ICAM-1, Fc Tag with the EC50 of 3.21 ng/ml.
Technical Parameters
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Species Human
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Source CHO
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Tag C-His
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Accession
P20701-1 (Y26-M1089)& P05107-1 (Q23-N700)
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Gene ID3683&3689
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Molecular Construction
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N-term
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ITGAL (Y26-M1089)
Accession # P20701-1 -
His
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C-term
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N-term
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ITGB2 (Q23-N700)
Accession # P05107-1 -
C-term
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Synonyms
ITGAL; Integrin Alpha-L; Prev. CD11A; HNA-5; LFA-1; Antigen CD11A (P180), Lymphocyte Function-Associated Antigen 1, Alpha Polypeptide; LFA1A; CDNA FLJ57691, Highly Similar To Integrin Alpha-L; Integrin, Alpha L (Antigen CD11A (P180), Lymphocyte Function-A
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Predicted Molecular Mass
124.1 kDa (ITGAL) & 80.2 kDa (ITGB2)
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Molecular Weight
Approximately 140-150 kDa (ITGAL) & 90-95 kDa (ITGB2), based on SDS-PAGE under non reducing (N) conditions, due to the glycosylation.
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Glycosylation
Yes
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Structure/Form
Monomer
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 7.5 with 10% trehalose as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (235 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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