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Integrin alpha M beta 2 Protein, Human (HEK293, His)

Customer Review

Based on 1 Customer Validation

Integrin α M β 2 (ITGAM/ITGB2) mediates adhesion of monocytes and granulocytes and promotes uptake of complement-coated particles and pathogens. As a receptor for iC3b and fibrinogen, it regulates neutrophil migration, contributes to CD177-PRTN3-mediated activation, and may influence phagocytosis-induced apoptosis. Integrin alpha M beta 2 Protein, Human (HEK293, His) is a recombinant protein dimer complex containing human-derived Integrin alpha M beta 2 protein, expressed by HEK293 , with C-His labeled tag. Integrin alpha M beta 2 Protein, Human (HEK293, His), has molecular weight of 140-160 kDa (ITGAM) & 90-100 kDa (ITGB2), respectively.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Integrin alpha M beta 2 Protein, Human (HEK293, His)
  • Experimental Validation Results for Integrin alpha M beta 2 Protein, Human (HEK293, His)
  • Integrin alpha M beta 2 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
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Biological Activity

Description

Integrin α M β 2 (ITGAM/ITGB2) mediates adhesion of monocytes and granulocytes and promotes uptake of complement-coated particles and pathogens. As a receptor for iC3b and fibrinogen, it regulates neutrophil migration, contributes to CD177-PRTN3-mediated activation, and may influence phagocytosis-induced apoptosis. Integrin alpha M beta 2 Protein, Human (HEK293, His) is a recombinant protein dimer complex containing human-derived Integrin alpha M beta 2 protein, expressed by HEK293 , with C-His labeled tag. Integrin alpha M beta 2 Protein, Human (HEK293, His), has molecular weight of 140-160 kDa (ITGAM) & 90-100 kDa (ITGB2), respectively.

Background

Integrin alpha M beta 2, also known as ITGAM/ITGB2, plays a crucial role in mediating adhesive interactions of monocytes, macrophages, and granulocytes, facilitating the uptake of complement-coated particles and pathogens. It serves as the receptor for the iC3b fragment of the third complement component and likely recognizes the R-G-D peptide in C3b. Additionally, this integrin complex acts as a receptor for fibrinogen, factor X, and ICAM1, recognizing specific peptides within fibrinogen gamma chain. ITGAM/ITGB2 regulates neutrophil migration and is essential for CD177-PRTN3-mediated activation of TNF-primed neutrophils. It may also contribute to the regulation of phagocytosis-induced apoptosis in extravasated neutrophils and play a role in mast cell development. Furthermore, in conjunction with TYROBP/DAP12, it is required in microglia to control the production of superoxide ions, promoting neuronal apoptosis during brain development. The interaction with various proteins, including JAM3 and THBD, highlights the multifaceted functions of Integrin alpha M beta 2 in cellular processes. Additionally, its association with complement factor H/CFH facilitates neutrophil adhesion to pathogens, contributing to pathogen clearance.

Verified Bioactivity

1.Immobilized Human Integrin alpha M beta 2, His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 13.7 ng/mL determined by ELISA.
2.Immobilized Human ITGAM&ITGB2, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 13.2 ng/ml determined by ELISA.
3.Measured by its ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with ICAM-1. The ED50 for this effect is 0.25-1.0 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with ICAM-1. The ED50 for this effect is 0.2800 μg/mL, corresponding to a specific activity is 3571.429 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    P11215-1 (F17-N1104)&P05107-1 (Q23-N700)

  • Gene ID
    3684&3689
  • Synonyms

    ITGAM; Human Neutrophil Antigen 4; Prev. CR3A; HNA-4; Prev. CD11B; Integrin, Alpha M (Complement Component Receptor 3, Alpha; Also Known As CD11b (P170), Macrophage Antigen Alpha Polypeptide); Integrin Alpha-M; Neutrophil Adherence Receptor Alpha-M Subuni

  • Molecular Weight

    Approximately 140-160 kDa (ITGAM) & 90-100 kDa (ITGB2), based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Structure/Form

    Heterodimer

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with ICAM-1. The ED50 for this effect is 0.2800 μg/mL, corresponding to a specific activity is 3571.429 units/mg.

  • Integrin alpha M beta 2 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Integrin alpha M beta 2 Protein, Human (HEK293, His)
  • Experimental Validation Results for Integrin alpha M beta 2 Protein, Human (HEK293, His)
  • Integrin alpha M beta 2 Protein, Human (HEK293, His) Product Image