Integrin alpha M beta 2 Protein, Human (HEK293, His)

Integrin α M β 2 (ITGAM/ITGB2) mediates adhesion of monocytes and granulocytes and promotes uptake of complement-coated particles and pathogens. As a receptor for iC3b and fibrinogen, it regulates neutrophil migration, contributes to CD177-PRTN3-mediated activation, and may influence phagocytosis-induced apoptosis. Integrin alpha M beta 2 Protein, Human (HEK293, His) is a recombinant protein dimer complex containing human-derived Integrin alpha M beta 2 protein, expressed by HEK293 , with C-His labeled tag. Integrin alpha M beta 2 Protein, Human (HEK293, His), has molecular weight of 140-160 kDa (ITGAM) & 90-100 kDa (ITGB2), respectively.

Integrin alpha M beta 2, also known as ITGAM/ITGB2, plays a crucial role in mediating adhesive interactions of monocytes, macrophages, and granulocytes, facilitating the uptake of complement-coated particles and pathogens. It serves as the receptor for the iC3b fragment of the third complement component and likely recognizes the R-G-D peptide in C3b. Additionally, this integrin complex acts as a receptor for fibrinogen, factor X, and ICAM1, recognizing specific peptides within fibrinogen gamma chain. ITGAM/ITGB2 regulates neutrophil migration and is essential for CD177-PRTN3-mediated activation of TNF-primed neutrophils. It may also contribute to the regulation of phagocytosis-induced apoptosis in extravasated neutrophils and play a role in mast cell development. Furthermore, in conjunction with TYROBP/DAP12, it is required in microglia to control the production of superoxide ions, promoting neuronal apoptosis during brain development. The interaction with various proteins, including JAM3 and THBD, highlights the multifaceted functions of Integrin alpha M beta 2 in cellular processes. Additionally, its association with complement factor H/CFH facilitates neutrophil adhesion to pathogens, contributing to pathogen clearance.

Immobilized Human Integrin alpha M beta 2, His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC₅₀ of 13.7 ng/mL determined by ELISA.

Human

HEK293

C-His

P11215-1 (F17-N1104)&P05107-1 (Q23-N700)

/&3689

140-160 kDa (ITGAM) & 90-100 kDa (ITGB2)

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.