ITK Protein, Human (Active, 269a.a, sf9, His, GST)

ITK protein is an important tyrosine kinase that regulates adaptive immune responses by affecting the development and function of T cells and NKT cells. Upon T cell receptor (TCR) activation, ITK undergoes LCK-mediated phosphorylation, leading to its activation. ITK Protein, Human (Active, 269a.a, sf9, His, GST) is the recombinant human-derived ITK, expressed by Sf9 insect cells, with His and GST labeled tag.

ITK protein, a crucial tyrosine kinase, plays an indispensable role in the regulation of the adaptive immune response by orchestrating the development, function, and differentiation of conventional T-cells and nonconventional NKT-cells. Upon activation of the T-cell receptor (TCR) by antigen-presenting cells (APC), a phosphorylation cascade ensues, leading to the recruitment of ITK to the cell membrane in close proximity to the stimulated TCR receptor. Subsequently, ITK undergoes phosphorylation by LCK, resulting in its autophosphorylation and full activation. Once activated, ITK phosphorylates PLCG1, activating this lipase and initiating the cleavage of its substrates. This process triggers the release of calcium from the endoplasmic reticulum into the cytoplasm, facilitating the translocation of the nuclear activator of activated T-cells (NFAT) into the nucleus for transcriptional regulation. Additionally, ITK phosphorylates essential adapter proteins like the linker for activation of T-cells/LAT protein and LCP2, recruiting a myriad of signaling molecules, including VAV1, ultimately leading to lymphokine production, T-cell proliferation, and differentiation. ITK is crucial for TCR-mediated calcium response in gamma-delta T-cells and may play a role in modulating the transcriptomic signature in the Vgamma2-positive subset of immature gamma-delta T-cells. Furthermore, ITK phosphorylates TBX21 at 'Tyr-530,' mediating its interaction with GATA3. This multifaceted involvement underscores the pivotal role of ITK in shaping diverse facets of the adaptive immune response.

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecencelabeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. When the protein concentration is 6.25 nM, the conversion rate is about 20%.

Human

Sf9 insect cells

N-His;N-GST

Q08881 (R352-L620)

3702

Full Length

Approximately 58 kDa, based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.