2. Recombinant Proteins
  3. Enzymes & Regulators Kinases
  4. Transferases (EC 2) Pseudokinases TK
  5. Janus kinase Jak
  6. Domain2_JAK3
  7. JAK3 Protein, Human (Biotinylated, Inactive, sf9, Avi-His)

JAK3 Protein, Human (Biotinylated, Inactive, sf9, Avi-His)

Cat. No.: HY-P705710
Handling Instructions Technical Support

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Species

Human
Source

Sf9 insect cells
Tag

C-His;C-Avi
Accession

P52333-1 (M511-D790)
Gene ID

3718
Molecular Construction
N-term
(R211-D790)
Accession # P52333
His
Avi
C-term
Protein Length

Partial
Conjugation

Biotin
Conjugate Method

The single lysine residue in Avitag is biotinylated through an enzymatic reaction.
Synonyms
Tyrosine-protein kinase JAK3; EC:2.7.10.2; Janus kinase 3 (JAK-3); Leukocyte janus kinase (L-JAK); JAK3
AA Sequence

MTFHKIPADSLEWHENLGHGSFTKIYRGCRHEVVDGEARKTEVLLKVMDAKHKNCMESFLEAASLMSQVSYRHLVLLHGVCMAGDSTMVQEFVHLGAIDMYLRKRGHLVPASWKLQVVKQLAYALNYLEDKGLPHGNVSARKVLLAREGADGSPPFIKLSDPGVSPAVLSLEMLTDRIPWVAPECLREAQTLSLEADKWGFGATVWEVFSGVTMPISALDPAKKLQFYEDRQQLPAPKWTELALLIQQCMAYEPVQRPSFRAVIRDLNSLISSDYELLSD

Predicted Molecular Mass
34 kDa
Purity

≥ 90%, as determined by reducing SDS-PAGE.
Documentation
Handling Instructions (2659 KB)

JAK3 Protein, Human (Biotinylated, Inactive, sf9, Avi-His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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