JAML/AMICA Protein, Human (HEK293, His)

JAML is a leukocyte plasma membrane transmembrane protein that critically regulates cell migration and activation by interacting with CXADR on neighboring cells. This interaction activates γ-δ T cells and induces downstream signaling through PI3 kinase and MAP kinase. JAML/AMICA Protein, Human (HEK293, His) is the recombinant human-derived JAML/AMICA protein, expressed by HEK293 , with C-His, C-6*His labeled tag.

JAML, a transmembrane protein residing on the plasma membrane of leukocytes, serves as a crucial regulator of cell migration and activation by engaging with CXADR, a receptor located on adjacent epithelial and endothelial cells. This interaction, pivotal for the activation of gamma-delta T-cells, a subset involved in tissue homeostasis and repair, induces downstream signaling events in response to epithelial CXADR-binding. JAML-mediated signaling, facilitated by PI3-kinase and MAP kinases, leads to T-cell proliferation and the production of cytokines and growth factors, fostering tissue repair. Additionally, JAML plays a pivotal role in controlling leukocyte transmigration within epithelial and endothelial tissues through adhesive interactions with CXADR. In its homodimeric form, JAML actively participates in leukocyte-endothelial cell adhesion, highlighting its multifaceted role in orchestrating immune responses and tissue repair processes. Furthermore, JAML's interaction with integrins ITGA4 and ITGB1 suggests a regulatory mechanism for leukocyte-endothelial cell adhesion by modulating JAML homodimerization.

Measured by the ability of the immobilized protein to support the adhesion of A549 Human non-small cell lung cancer cells. When 5×10⁴ cells/well are added to rhAMICA/Fc Chimera coated plates (10 µg/mL, 100 µL/well), approximately 87.96% will adhere after 1 hour at 37℃.

Human

HEK293

C-6*His

Q86YT9-1/NP_001091996.1 (L20-L275)

120425  [NCBI]

Extracellular Domain

LNDLNVSPPELTVHVGDSALMGCVFQSTEDKCIFKIDWTLSPGEHAKDEYVLYYYSNLSVPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYICEIRLKGESQVFKKAVVLHVLPEEPKELMVHVGGLIQMGCVFQSTEVKHVTKVEWIFSGRRAKEEIVFRYYHKLRMSVEYSQSWGHFQNRVNLVGDIFRNDGSIMLQGVRESDGGNYTCSIHLGNLVFKKTIVLHVSPEEPRTLVTPAALRPLVLGGNQL

Approximately 39 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.