JAML/AMICA Protein, Human (HEK293, His)
Based on 1 Customer Validation
JAML is a leukocyte plasma membrane transmembrane protein that critically regulates cell migration and activation by interacting with CXADR on neighboring cells. This interaction activates γ-δ T cells and induces downstream signaling through PI3 kinase and MAP kinase. JAML/AMICA Protein, Human (HEK293, His) is the recombinant human-derived JAML/AMICA protein, expressed by HEK293 , with C-His, C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
JAML is a leukocyte plasma membrane transmembrane protein that critically regulates cell migration and activation by interacting with CXADR on neighboring cells. This interaction activates γ-δ T cells and induces downstream signaling through PI3 kinase and MAP kinase. JAML/AMICA Protein, Human (HEK293, His) is the recombinant human-derived JAML/AMICA protein, expressed by HEK293 , with C-His, C-6*His labeled tag.
Background
JAML, a transmembrane protein residing on the plasma membrane of leukocytes, serves as a crucial regulator of cell migration and activation by engaging with CXADR, a receptor located on adjacent epithelial and endothelial cells. This interaction, pivotal for the activation of gamma-delta T-cells, a subset involved in tissue homeostasis and repair, induces downstream signaling events in response to epithelial CXADR-binding. JAML-mediated signaling, facilitated by PI3-kinase and MAP kinases, leads to T-cell proliferation and the production of cytokines and growth factors, fostering tissue repair. Additionally, JAML plays a pivotal role in controlling leukocyte transmigration within epithelial and endothelial tissues through adhesive interactions with CXADR. In its homodimeric form, JAML actively participates in leukocyte-endothelial cell adhesion, highlighting its multifaceted role in orchestrating immune responses and tissue repair processes. Furthermore, JAML's interaction with integrins ITGA4 and ITGB1 suggests a regulatory mechanism for leukocyte-endothelial cell adhesion by modulating JAML homodimerization.
Verified Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of A549 Human non-small cell lung cancer cells. When 5×104 cells/well are added to rhAMICA/Fc Chimera coated plates (10 µg/mL, 100 µL/well), approximately 87.96% will adhere after 1 hour at 37℃.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
Q86YT9-1/NP_001091996.1 (L20-L275)
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Molecular Construction
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N-term
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JAML (L20-L275)
Accession # Q86YT9-1/NP_001091996.1 -
6*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
JAML; Adhesion Molecule, Interacts With CXADR Antigen 1; Prev. AMICA1; Dendritic-Cell Specific Protein CREA7-4; Junctional Adhesion Molecule-Like; Dendritic Cell-Specific Protein CREA7-1; Gm638; Adhesion Molecule AMICA; AMICA; CREA7-1; Adhesion Molecule I
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AA Sequence
LNDLNVSPPELTVHVGDSALMGCVFQSTEDKCIFKIDWTLSPGEHAKDEYVLYYYSNLSVPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYICEIRLKGESQVFKKAVVLHVLPEEPKELMVHVGGLIQMGCVFQSTEVKHVTKVEWIFSGRRAKEEIVFRYYHKLRMSVEYSQSWGHFQNRVNLVGDIFRNDGSIMLQGVRESDGGNYTCSIHLGNLVFKKTIVLHVSPEEPRTLVTPAALRPLVLGGNQL
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Molecular Weight
Approximately 38-43 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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