1. Recombinant Proteins
  2. Receptor Proteins
  3. Cell Adhesion Molecules (CAMs)
  4. Immunoglobulin-like Cell Adhesion Molecules
  5. Junctional Adhesion Molecule-Like Protein (JAML)
  6. JAML/AMICA Protein, Human (HEK293, His)

JAML/AMICA Protein, Human (HEK293, His)

Cat. No.: HY-P75895
Handling Instructions

JAML is a leukocyte plasma membrane transmembrane protein that critically regulates cell migration and activation by interacting with CXADR on neighboring cells. This interaction activates γ-δ T cells and induces downstream signaling through PI3 kinase and MAP kinase. JAML/AMICA Protein, Human (HEK293, His) is the recombinant human-derived JAML/AMICA protein, expressed by HEK293 , with C-His, C-6*His labeled tag. The total length of JAML/AMICA Protein, Human (HEK293, His) is 256 a.a., with molecular weight of ~39 kDa.

For research use only. We do not sell to patients.

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Description

JAML is a leukocyte plasma membrane transmembrane protein that critically regulates cell migration and activation by interacting with CXADR on neighboring cells. This interaction activates γ-δ T cells and induces downstream signaling through PI3 kinase and MAP kinase. JAML/AMICA Protein, Human (HEK293, His) is the recombinant human-derived JAML/AMICA protein, expressed by HEK293 , with C-His, C-6*His labeled tag. The total length of JAML/AMICA Protein, Human (HEK293, His) is 256 a.a., with molecular weight of ~39 kDa.

Background

JAML, a transmembrane protein residing on the plasma membrane of leukocytes, serves as a crucial regulator of cell migration and activation by engaging with CXADR, a receptor located on adjacent epithelial and endothelial cells. This interaction, pivotal for the activation of gamma-delta T-cells, a subset involved in tissue homeostasis and repair, induces downstream signaling events in response to epithelial CXADR-binding. JAML-mediated signaling, facilitated by PI3-kinase and MAP kinases, leads to T-cell proliferation and the production of cytokines and growth factors, fostering tissue repair. Additionally, JAML plays a pivotal role in controlling leukocyte transmigration within epithelial and endothelial tissues through adhesive interactions with CXADR. In its homodimeric form, JAML actively participates in leukocyte-endothelial cell adhesion, highlighting its multifaceted role in orchestrating immune responses and tissue repair processes. Furthermore, JAML's interaction with integrins ITGA4 and ITGB1 suggests a regulatory mechanism for leukocyte-endothelial cell adhesion by modulating JAML homodimerization.

Biological Activity

Measured by the ability of the immobilized protein to support the adhesion of A549 Human non-small cell lung cancer cells. When 5×104 cells/well are added to rhAMICA/Fc Chimera coated plates (10 µg/mL, 100 µL/well), approximately 87.96% will adhere after 1 hour at 37℃.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q86YT9/NP_001091996.1 (L20-L275)

Gene ID
Molecular Construction
N-term
JAML (L20-L275)
Accession # Q86YT9/NP_001091996.1
6*His
C-term
Synonyms
Junctional adhesion molecule-like; JAML; AMICA1; CREA7-1
AA Sequence

LNDLNVSPPELTVHVGDSALMGCVFQSTEDKCIFKIDWTLSPGEHAKDEYVLYYYSNLSVPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYICEIRLKGESQVFKKAVVLHVLPEEPKELMVHVGGLIQMGCVFQSTEVKHVTKVEWIFSGRRAKEEIVFRYYHKLRMSVEYSQSWGHFQNRVNLVGDIFRNDGSIMLQGVRESDGGNYTCSIHLGNLVFKKTIVLHVSPEEPRTLVTPAALRPLVLGGNQL

Molecular Weight

Approximately 39 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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JAML/AMICA Protein, Human (HEK293, His)
Cat. No.:
HY-P75895
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