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KRT18 Protein, Human (HEK293, His)

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The KRT18 protein is a heterotetramer with KRT8 that has multiple roles, including uptake of thrombin-antithrombin complexes and assisting in the delivery of mutated CFTR to the plasma membrane. It cooperates with KRT8 in IL-6-mediated barrier protection and interacts with PLEC isoform 1C. KRT18 Protein, Human (HEK293, His) is the recombinant human-derived KRT18 protein, expressed by HEK293 , with N-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • KRT18 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The KRT18 protein is a heterotetramer with KRT8 that has multiple roles, including uptake of thrombin-antithrombin complexes and assisting in the delivery of mutated CFTR to the plasma membrane. It cooperates with KRT8 in IL-6-mediated barrier protection and interacts with PLEC isoform 1C. KRT18 Protein, Human (HEK293, His) is the recombinant human-derived KRT18 protein, expressed by HEK293 , with N-10*His labeled tag.

Background

The KRT18 Protein plays diverse roles in cellular processes, including the uptake of thrombin-antithrombin complexes by hepatic cells and the reorganization of filaments when phosphorylated. It actively participates in the delivery of mutated CFTR to the plasma membrane and collaborates with KRT8 in interleukin-6 (IL-6)-mediated barrier protection. As a heterotetramer of two type I and two type II keratins, KRT18 forms a complex with KRT8, and interacts with PLEC isoform 1C when in a heterodimer with KRT8. Additionally, KRT18 engages in interactions with the thrombin-antithrombin complex and exhibits connections with various proteins such as PNN, mutated CFTR, YWHAE, YWHAH, YWHAZ (when phosphorylated), DNAJB6, TCHP, TRADD, FAM83H, EPPK1, PKP1, and PKP2. The multifaceted interactions and functions of KRT18 highlight its involvement in fundamental cellular activities and its contribution to molecular pathways governing cell structure and signaling.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag N-10*His
  • Accession

    P05783 (S2-H430)

  • Gene ID
    3875  [NCBI]
  • Molecular Construction
    • N-term
    • 10*His
    • KRT18 (S2-H430)
      Accession # P05783
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    KRT18; CYK18; Keratin 18; K18; Cell Proliferation-Inducing Gene 46 Protein; Cytokeratin 18; Keratin, Type I Cytoskeletal 18; Cytokeratin-18; Keratin 18, Type I; KRT18 Protein; CK-18; Keratin-18

  • AA Sequence

    SFTTRSTFSTNYRSLGSVQAPSYGARPVSSAASVYAGAGGSGSRISVSRSTSFRGGMGSGGLATGIAGGLAGMGGIQNEKETMQSLNDRLASYLDRVRSLETENRRLESKIREHLEKKGPQVRDWSHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRH

  • Predicted Molecular Mass

    50.8 kDa

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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