KRT8 Protein, Human (Baculovirus, His-Myc)

The KRT8 protein, in cooperation with KRT19, critically links the contractile apparatus of the striated rib muscle to dystrophin. This heterotetramer consists of two type I keratins and two type II keratins, forming a heterodimer with KRT18. KRT8 Protein, Human (Baculovirus, His-Myc) is the recombinant human-derived KRT8 protein, expressed by Sf9 insect cells , with N-10*His, C-Myc labeled tag. The total length of KRT8 Protein, Human (Baculovirus, His-Myc) is 483 a.a., with molecular weight of ~57.6 kDa.

The KRT8 protein, in conjunction with KRT19, plays a crucial role in connecting the contractile apparatus to dystrophin at the costameres of striated muscle. This heterotetramer consists of two type I and two type II keratins and forms a heterodimer with KRT18. Additionally, KRT8 associates with KRT20 and interacts with PLEC isoform 1C when in a heterodimer with KRT18. It forms complexes with several other proteins, including PNN, DMD (when associated with KRT19), TCHP, APEX1, GPER1, and EPPK1. Furthermore, KRT8 interacts with the desmosomal proteins PKP1 and PKP2. These diverse protein interactions highlight the multifaceted role of KRT8 in contributing to the structural integrity and functional dynamics of the cellular cytoskeleton in various cellular contexts.

Human

Sf9 insect cells

N-10*His;C-Myc

P05787 (M1-K483)

3856  [NCBI]

MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK

Approximately 57.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.