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  4. LAIR-1 LAIR-1/CD305
  5. LAIR1 Protein, Human (HEK293, His)

LAIR1 Protein, Human (HEK293, His)

Cat. No.: HY-P72529
Handling Instructions

LAIR1 protein has a negative regulatory effect on NK cells, B cells and T cells. It activates the phosphatases PTPN6 and PTPN11, inhibits calcium increases in B cells, and regulates cytokine production in CD4+ T cells. LAIR1 Protein, Human (HEK293, His) is the recombinant human-derived LAIR1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of LAIR1 Protein, Human (HEK293, His) is 142 a.a., with molecular weight of ~28 kDa.

For research use only. We do not sell to patients.

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Description

LAIR1 protein has a negative regulatory effect on NK cells, B cells and T cells. It activates the phosphatases PTPN6 and PTPN11, inhibits calcium increases in B cells, and regulates cytokine production in CD4+ T cells. LAIR1 Protein, Human (HEK293, His) is the recombinant human-derived LAIR1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of LAIR1 Protein, Human (HEK293, His) is 142 a.a., with molecular weight of ~28 kDa.

Background

The LAIR1 Protein serves as an inhibitory receptor, exerting a constitutive negative regulatory influence on the cytolytic function of natural killer (NK) cells, B-cells, and T-cells. Upon activation through tyrosine phosphorylation, it recruits and activates phosphatases PTPN6 and PTPN11. LAIR1 also dampens the increase in intracellular calcium triggered by B-cell receptor ligation. Beyond its dependency on SH2-containing phosphatases, it independently modulates cytokine production in CD4+ T-cells, suppressing IL2 and IFNG while promoting the secretion of transforming growth factor beta. Additionally, LAIR1 down-regulates IgG and IgE production in B-cells and hinders the secretion of IL8, IL10, and TNF. In myeloid leukemia cell lines, LAIR1 inhibits proliferation, induces apoptosis, and prevents nuclear translocation of NF-kappa-B p65 subunit/RELA, along with the phosphorylation of I-kappa-B alpha/CHUK. Moreover, LAIR1 inhibits the differentiation of peripheral blood precursors into dendritic cells. It interacts with the SH2 domains of tyrosine-protein phosphatases PTPN6 and PTPN11, with the interaction with PTPN6 being constitutive. Furthermore, LAIR1 binds with high affinity to extracellular matrix collagens, highlighting the functional importance of this interaction.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q6GTX8 (Q22-H163)

Gene ID
Molecular Construction
N-term
LAIR1 (Q22-H163)
Accession # Q6GTX8
6*His
C-term
Synonyms
Leukocyte-Associated Immunoglobulin-Like Receptor 1; LAIR-1; hLAIR1; CD305
AA Sequence

QEEDLPRPSISAEPGTVIPLGSHVTFVCRGPVGVQTFRLERESRSTYNDTEDVSQASPSESEARFRIDSVSEGNAGPYRCIYYKPPKWSEQSDYLELLVKETSGGPDSPDTEPGSSAGPTQRPSDNSHNEHAPASQGLKAEH

Molecular Weight

Approximately 28 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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LAIR1 Protein, Human (HEK293, His)
Cat. No.:
HY-P72529
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