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LECT1 Protein, Human (HEK293, Fc)

Cat. No.: HY-P75906
COA Handling Instructions

LECT1 Protein, a bifunctional growth regulator, stimulates cultured chondrocyte growth with FGF, promoting cartilage expansion. Conversely, it inhibits vascular endothelial cell growth, contrasting its effect on endothelial proliferation. This dual role may coordinate cartilage growth and vascular invasion during endochondral bone development, where LECT1 is implicated in rapid cartilage growth preceding bone replacement. Additionally, LECT1 acts as an antiangiogenic factor in cardiac valves, suppressing neovascularization and highlighting its intricate involvement in chondrogenesis and angiogenesis regulation. LECT1 Protein, Human (HEK293, Fc) is the recombinant human-derived LECT1 protein, expressed by HEK293, with N-hFc labeled tag. The total length of LECT1 Protein, Human (HEK293, Fc) is 119 a.a., with molecular weight of ~42.1 kDa.

For research use only. We do not sell to patients.

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Description

LECT1 Protein, a bifunctional growth regulator, stimulates cultured chondrocyte growth with FGF, promoting cartilage expansion. Conversely, it inhibits vascular endothelial cell growth, contrasting its effect on endothelial proliferation. This dual role may coordinate cartilage growth and vascular invasion during endochondral bone development, where LECT1 is implicated in rapid cartilage growth preceding bone replacement. Additionally, LECT1 acts as an antiangiogenic factor in cardiac valves, suppressing neovascularization and highlighting its intricate involvement in chondrogenesis and angiogenesis regulation. LECT1 Protein, Human (HEK293, Fc) is the recombinant human-derived LECT1 protein, expressed by HEK293, with N-hFc labeled tag. The total length of LECT1 Protein, Human (HEK293, Fc) is 119 a.a., with molecular weight of ~42.1 kDa.

Background

The LECT1 protein functions as a bifunctional growth regulator, exhibiting a dual role in cellular growth regulation. It stimulates the growth of cultured chondrocytes in the presence of basic fibroblast growth factor (FGF), suggesting a role in promoting the expansion of cartilage. Conversely, LECT1 inhibits the growth of cultured vascular endothelial cells, highlighting its contrasting effect on endothelial cell proliferation. This bifunctional nature may contribute to the coordinated regulation of cartilage growth and vascular invasion during endochondral bone development, where LECT1 is implicated in the rapid growth of cartilage preceding its replacement by bone. Moreover, LECT1 serves as an antiangiogenic factor in cardiac valves, suppressing neovascularization by inhibiting in vitro tube formation and the mobilization of endothelial cells. These multifaceted activities underscore LECT1's intricate involvement in the regulation of both chondrogenesis and angiogenesis processes.

Species

Human

Source

HEK293

Tag

N-hFc

Accession

O75829 (E215-V333)

Gene ID
Molecular Construction
N-term
hFc
LECT1 (E215-V333)
Accession # O75829
C-term
Synonyms
Leukocyte cell-derived chemotaxin 1; Chondromodulin; CH-SP; CNMD; CHMI; LECT1
Molecular Weight

Approximately 42.1 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

LECT1 Protein, Human (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
LECT1 Protein, Human (HEK293, Fc)
Cat. No.:
HY-P75906
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