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LEDGF p52 Protein, Human

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LEDGF p52 protein is involved in essential functions, such as DNA-binding and chromatin binding activity. It positively regulates transcription and plays a role in mRNA 5'-splice site recognition. The protein is located in heterochromatin, the nuclear periphery, and the nucleoplasm. It is ubiquitously expressed in various tissues, including the brain (RPKM 41.2), ovary (RPKM 33.4), and 24 other tissues. LEDGF p52 Protein, Human is the recombinant human-derived LEDGF p52 protein, expressed by E. coli , with tag free.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • LEDGF p52 Protein, Human Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

LEDGF p52 protein is involved in essential functions, such as DNA-binding and chromatin binding activity. It positively regulates transcription and plays a role in mRNA 5'-splice site recognition. The protein is located in heterochromatin, the nuclear periphery, and the nucleoplasm. It is ubiquitously expressed in various tissues, including the brain (RPKM 41.2), ovary (RPKM 33.4), and 24 other tissues. LEDGF p52 Protein, Human is the recombinant human-derived LEDGF p52 protein, expressed by E. coli , with tag free.

Background

The LEDGF p52 protein is involved in various essential functions, including DNA-binding transcription factor binding activity, chromatin binding activity, and transcription coactivator activity. It plays a role in mRNA 5'-splice site recognition and positively regulates transcription by RNA polymerase II. The protein is located in heterochromatin, the nuclear periphery, and the nucleoplasm. It exhibits ubiquitous expression in multiple tissues, including the brain (RPKM 41.2), ovary (RPKM 33.4), and 24 other tissues.

Verified Bioactivity

Measured in a cell proliferation assay using SH-SY5Y cells. The ED50 for this effect is 5.323 μg/mL.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    O75475-2/NP_066967 (M1-Q333)

  • Gene ID
    11168  [NCBI]
  • Molecular Construction
    • N-term
    • LEDGF p52 (M1-Q333)
      Accession # O75475-2/NP_066967
    • C-term
  • Protein Length

    Full Length of Isoform-2

  • Synonyms

    PSIP1; PC4 And SFRS1 Interacting Protein 1; Prev. PSIP2; CLL-Associated Antigen KW-7; Lens Epithelium-Derived Growth Factor; PC4 And SFRS1 Interacting Protein 2; LEDGF; Transcriptional Coactivator P52/P75; DFS70; Transcriptional Coactivator P75/P52; PC4 A

  • AA Sequence

    MTRDFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHETAFLGPKDIFPYSENKEKYGKPNKRKGFNEGLWEIDNNPKVKFSSQQAATKQSNASSDVEVEEKETSVSKEDTDHEEKASNEDVTKAVDITTPKAARRGRKRKAEKQVETEEAGVVTTATASVNLKVSPKRGRPAATEVKIPKPRGRPKMVKQPCPSESDIITEEDKSKKKGQEEKQPKKQPKKDEEGQKEEDKPRKEPDKKEGKKEVESKRKNLAKTGVTSTSDSEEEGDDQEGEKKRKGGRNFQTAHRRNMLKGQHEKEAADRKRKQEEQMETEHQTTCNLQ

  • Predicted Molecular Mass

    37.7 kDa

  • Molecular Weight

    Approximately 52 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 200 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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