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LILRA4/ILT-7/CD85g Protein, Human (HEK293, His)

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Based on 1 Customer Validation

The LILRA4/ILT-7/CD85g protein is a coreceptor that critically limits the innate immune response during viral infection through FCER1G signaling.It negatively regulates TLR7 and TLR9 signaling and reduces IFNA1, IFNA2, IFNA4, IFNB1, and TNF production in dendritic cells exposed to influenza virus or CpG dinucleotides.LILRA4/ILT-7/CD85g Protein, Human (HEK293, His) is the recombinant human-derived LILRA4/ILT-7/CD85g protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for LILRA4/ILT-7/CD85g Protein, Human (HEK293, His)
  • Experimental Validation Results for LILRA4/ILT-7/CD85g Protein, Human (HEK293, His)
  • Experimental Validation Results for LILRA4/ILT-7/CD85g Protein, Human (HEK293, His)
  • LILRA4/ILT-7/CD85g Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

The LILRA4/ILT-7/CD85g protein is a coreceptor that critically limits the innate immune response during viral infection through FCER1G signaling.It negatively regulates TLR7 and TLR9 signaling and reduces IFNA1, IFNA2, IFNA4, IFNB1, and TNF production in dendritic cells exposed to influenza virus or CpG dinucleotides.LILRA4/ILT-7/CD85g Protein, Human (HEK293, His) is the recombinant human-derived LILRA4/ILT-7/CD85g protein, expressed by HEK293 , with C-His labeled tag.

Background

LILRA4/ILT-7/CD85g protein functions as a coreceptor, exerting a pivotal role in limiting innate immune responses during viral infections, with signaling occurring through FCER1G. It acts as a negative regulator of TLR7 and TLR9 signaling cascades, demonstrated by its ability to down-regulate the production of IFNA1, IFNA2, IFNA4, IFNB1, and TNF in plasmacytoid dendritic cells exposed to influenza virus or CpG dinucleotides. Additionally, LILRA4/ILT-7/CD85g negatively modulates interferon production in response to interaction with BST2 on HIV-1 infected cells. The protein activates a signaling cascade in conjunction with FCER1G, leading to the phosphorylation of Src family and Syk kinases and subsequent mobilization of intracellular Ca(2+). Notably, LILRA4/ILT-7/CD85g does not interfere with the differentiation of plasmacytoid dendritic cells into antigen-presenting cells. The interaction between LILRA4/ILT-7/CD85g and FCER1G stabilizes the expression of both proteins at the cell membrane, emphasizing its crucial regulatory role in the innate immune response to viral pathogens.

Verified Bioactivity

1.Measured by its binding ability in a functional ELISA. Immobilized Human LILRA4 at 1 μg/mL (100 μL/well) can bind biotinylated Human ANGPTL7. The ED50 for this effect is 58.08 ng/mL.
2.Loaded Daxdilimab (FUT8-KO) (HY-P99226A) on ProA biosensor, can bind LILRA4/ILT-7/CD85g  Protein, Human (HEK293, His) with an affinity constant of 8.548E-08 M as  determined in BLI assay.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Human LILRA4 at 1 μg/mL (100 μL/well) can bind biotinylated Human ANGPTL7. The ED50 for this effect is 58.08 ng/mL.

  • Bioactivity - BLI

    Bioactivity - BLI

    Loaded Daxdilimab (FUT8-KO) (HY-P99226A) on ProA biosensor, can bind LILRA4/ILT-7/CD85g  Protein, Human (HEK293, His) with an affinity constant of 8.548E-08 M as  determined in BLI assay.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    P59901-1 (E24-N446)

  • Gene ID
    23547  [NCBI]
  • Molecular Construction
    • N-term
    • LILRA4 (E24-N446)
      Accession # P59901
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    LILRA4; CD85 Antigen-Like Family Member G; Leukocyte Immunoglobulin Like Receptor A4; Immunoglobulin-Like Transcript 7; ILT7; Leucocyte Ig-Like Receptor A4; CD85g; Leukocyte Immunoglobulin-Like Receptor, Subfamily A (Without TM Domain), Member 4; Leukocyt

  • AA Sequence

    ENLLKPILWAEPGPVITWHNPVTIWCQGTLEAQGYRLDKEGNSMSRHILKTLESENKVKLSIPSMMWEHAGRYHCYYQSPAGWSEPSDPLELVVTAYSRPTLSALPSPVVTSGVNVTLRCASRLGLGRFTLIEEGDHRLSWTLNSHQHNHGKFQALFPMGPLTFSNRGTFRCYGYENNTPYVWSEPSDPLQLLVSGVSRKPSLLTLQGPVVTPGENLTLQCGSDVGYIRYTLYKEGADGLPQRPGRQPQAGLSQANFTLSPVSRSYGGQYRCYGAHNVSSEWSAPSDPLDILIAGQISDRPSLSVQPGPTVTSGEKVTLLCQSWDPMFTFLLTKEGAAHPPLRLRSMYGAHKYQAEFPMSPVTSAHAGTYRCYGSRSSNPYLLSHPSEPLELVVSGATETLNPAQKKSDSKTAPHLQDYTVEN

  • Molecular Weight

    Approximately 60-75 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Human LILRA4 at 1 μg/mL (100 μL/well) can bind biotinylated Human ANGPTL7. The ED50 for this effect is 58.08 ng/mL.

  • 1/16

    MedChemExpress Validation
     Loaded Daxdilimab (FUT8-KO) (HY-P99226A) on ProA biosensor, can bind LILRA4/ILT-7/CD85g  Protein, Human (HEK293, His) with an affinity constant of 8.548E-08 M as  determined in BLI assay.

  • LILRA4/ILT-7/CD85g Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for LILRA4/ILT-7/CD85g Protein, Human (HEK293, His)
  • Experimental Validation Results for LILRA4/ILT-7/CD85g Protein, Human (HEK293, His)
  • Experimental Validation Results for LILRA4/ILT-7/CD85g Protein, Human (HEK293, His)
  • LILRA4/ILT-7/CD85g Protein, Human (HEK293, His) Product Image