2. Protéines recombinantes
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. LMTK3 Protein, Mouse (sf9, GST)

LMTK3 Protein, Mouse (sf9, GST) is the recombinant mouse-derived LMTK3, expressed by Sf9 insect cells , with GST labeled tag. ,

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Description

LMTK3 Protein, Mouse (sf9, GST) is the recombinant mouse-derived LMTK3, expressed by Sf9 insect cells , with GST labeled tag. ,
Background

LMTK3 is a protein kinase that phosphorylates ESR1 in vitro and protects it from proteasomal degradation. It may also regulate ESR1 levels indirectly via a PKC-AKT-FOXO3 pathway, where it reduces PKC activity and AKT phosphorylation, thereby enhancing the binding of the transcriptional activator FOXO3 to the ESR1 promoter and increasing ESR1 transcription (By similarity). Additionally, LMTK3 is involved in the endocytic trafficking of N-methyl-D-aspartate receptors (NMDAR) in neurons (PubMed:24760852).
Species

Mouse
Source

Sf9 insect cells
Tag

GST
Accession

Q5XJV6 (K63-A484)
Gene ID

381983
Protein Length

Partial
Synonyms
Aatyk3
AA Sequence

KRGDVRFKEFENPEGEDCSGEYTPPAEETSSSQSLPDVYILPLAEVSLPMPAPQPPHSDISTPLGLSRQHLSYLQEIGSGWFGKVILGEVFSDYSPAQVVVKELRASAGPLEQRKFISEAQPYRSLQHPNVLQCLGVCVETLPFLLIMEFCQLGDLKRYLRAQRPPEGMSPELPPRDLRTLQRMGLEIARGLAHLHSHNYVHSDLALRNCLLTSDLTVRIGDYGLAHSNYKEDYYLTPERLWVPLRWAAPELLGELHGSFVLVDQSRESNVWSLGVTLWELFEFGAQPYRHLSDEEVLAFVVRQQHVKLARPRLKLPYADYWYDILQSCWRPPAQRPSASDLQLQLTYLLSERPPRPPPPPPPPRDGPFPWPWPPSHSAPRPGTLSSQFPLLDGFPGADPDDVLTVTESSRGLNLECLWEKA

Predicted Molecular Mass
75 kDa
Pureté

≥ 85%, as determined by reducing SDS-PAGE.
Documentation
Instruction de manipulation (2659 KB)

LMTK3 Protein, Mouse (sf9, GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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