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LRP-1 Cluster II Protein, Human (HEK293, His)

3 Cited Publications
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Based on 3 publication(s) in Google Scholar

LRP-1 Cluster II protein is involved in endocytosis and phagocytosis of apoptotic cells. The LRP-1 Cluster II protein also serves as a receptor for Pseudomonas aeruginosa exotoxin A during microbial infection. LRP-1 Cluster II Protein, Human (HEK293, His) is the recombinant human-derived LRP-1 Cluster II protein, expressed by HEK293 , with C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for LRP-1 Cluster II Protein, Human (HEK293, His)
  • Experimental Validation Results for LRP-1 Cluster II Protein, Human (HEK293, His)
  • LRP-1 Cluster II Protein, Human (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

LRP-1 Cluster II protein is involved in endocytosis and phagocytosis of apoptotic cells. The LRP-1 Cluster II protein also serves as a receptor for Pseudomonas aeruginosa exotoxin A during microbial infection. LRP-1 Cluster II Protein, Human (HEK293, His) is the recombinant human-derived LRP-1 Cluster II protein, expressed by HEK293 , with C-10*His labeled tag.

Background

LRP-1 Cluster II Protein emerges as a multifunctional endocytic receptor, playing pivotal roles in both endocytosis and the phagocytosis of apoptotic cells. Its significance extends to essential functions in early embryonic development and cellular lipid homeostasis. Additionally, LRP-1 Cluster II is actively involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the localized metabolism of complexes formed between plasminogen activators and their endogenous inhibitors. Acting as an LRPAP1 alpha-2-macroglobulin receptor, it further participates in various cellular events, including the endocytosis and subsequent spread of TAU/MAPT. Moreover, LRP-1 Cluster II may modulate critical cellular processes such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling, and neurotransmission. Notably, in microbial infection, the protein functions as a receptor for Pseudomonas aeruginosa exotoxin A, highlighting its diverse and intricate roles in cellular physiology and host-pathogen interactions.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant LRP-1 Cluster II Protein is immobilized at 50 ng/mL (100 μL/well) can bind Recombinant Human LRPAP (HY-P76478). The ED50 for this effect is <2.5 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When Recombinant LRP-1 Cluster II Protein is immobilized at 50 ng/mL (100 μL/well) can bind Recombinant Human LRPAP (HY-P76478). The ED50 for this effect is 2.302 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    Q07954-1 (R786-L1165)

  • Gene ID
    4035
  • Molecular Construction
    • N-term
    • LRP-1 Cluster II (R786-L1165)
      Accession # Q07954-1
    • 10*His
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    LRP1; Apolipoprotein E Receptor; Prev. A2MR; TbetaR-V/LRP-1/IGFBP-3 Receptor; Prev. APR; Type V Tgf-Beta Receptor; APOER; Alternative Protein LRP1; Alpha-2-Macroglobulin Receptor; CD91 Antigen; IGFBP3R1; LRP1 Protein; IGFBP-3R; IGFBP3R; LRP1A; TGFBR5; CD9

  • AA Sequence

    RPPIFEIRMYDAQQQQVGTNKCRVNNGGCSSLCLATPGSRQCACAEDQVLDADGVTCLANPSYVPPPQCQPGEFACANSRCIQERWKCDGDNDCLDNSDEAPALCHQHTCPSDRFKCENNRCIPNRWLCDGDNDCGNSEDESNATCSARTCPPNQFSCASGRCIPISWTCDLDDDCGDRSDESASCAYPTCFPLTQFTCNNGRCININWRCDNDNDCGDNSDEAGCSHSCSSTQFKCNSGRCIPEHWTCDGDNDCGDYSDETHANCTNQATRPPGGCHTDEFQCRLDGLCIPLRWRCDGDTDCMDSSDEKSCEGVTHVCDPSVKFGCKDSARCISKAWVCDGDNDCEDNSDEENCESLACRPPSHPCANNTSVCLPPDKL

  • Predicted Molecular Mass

    41.5 kDa

  • Molecular Weight

    Approximately 65-80 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant LRP-1 Cluster II Protein is immobilized at 50 ng/mL (100 μL/well) can bind Recombinant Human LRPAP (HY-P76478). The ED50 for this effect is 2.302 ng/mL.

  • LRP-1 Cluster II Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for LRP-1 Cluster II Protein, Human (HEK293, His)
  • Experimental Validation Results for LRP-1 Cluster II Protein, Human (HEK293, His)
  • LRP-1 Cluster II Protein, Human (HEK293, His) Product Image