MAX Protein, Human (sf9, His-GST)

MAX protein is a transcriptional regulator that forms a complex with MYC to promote activation, or a complex with MAD to cause inhibition, binding to the core sequence 5'-CAC[GA]TG-3'. MAX acts as a repressor protein that recruits chromatin remodeling complexes with H3 "Lys-9" histone methyltransferase activity. MAX Protein, Human (sf9, His-GST) is the recombinant human-derived MAX protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

MAX, a transcription regulator, forms a sequence-specific DNA-binding protein complex either with MYC, promoting transcriptional activation, or with MAD, leading to transcriptional repression, recognizing the core sequence 5'-CAC[GA]TG-3'. MAX may act as a transcriptional repressor by recruiting a chromatin remodeling complex with H3 'Lys-9' histone methyltransferase activity and repressing MYC transcriptional activity from E-box elements. Efficient DNA binding requires dimerization with another bHLH protein. It binds DNA as a heterodimer with either MYC or MAD. MAX is part of the E2F6.com-1 complex during the G0 phase, along with various other components. It is also a component of some MLL1/MLL complexes, contributing to the regulation of gene expression. MAX interacts with SPAG9, and when in a heterodimer with MYC, it interacts with ABI1, potentially enhancing MYC:MAX transcriptional activity. MAX's versatility in forming complexes with different partners underscores its role as a key modulator of gene expression.

Human

Sf9 insect cells

N-His;N-GST

P61244-1/NP_002373 (M1-S160)

4149  [NCBI]

Full Length of Isoform-1

MSDNDDIEVESDEEQPRFQSAADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS

Approximately 44.47 kDa,based on SDS-PAGE under reducing conditions.

≥ 85%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10% Glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.