MCP-1/CCL2 Protein, Cynomolgus (HEK293, His)
MCP-1/CCL2 protein activates CCR2 and induces chemotaxis and calcium ion mobilization. It attracts monocytes and basophils, not neutrophils or eosinophils. It also contributes to neuropathic pain and enhances synaptic transmission in dopamine neurons. MCP-1/CCL2 exists as a monomer or homodimer and binds to endothelial cells through proteoglycans. It interacts with TNFAIP6 via its Link domain. MCP-1/CCL2 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived MCP-1/CCL2 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Cynomolgus
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
MCP-1/CCL2 protein activates CCR2 and induces chemotaxis and calcium ion mobilization. It attracts monocytes and basophils, not neutrophils or eosinophils. It also contributes to neuropathic pain and enhances synaptic transmission in dopamine neurons. MCP-1/CCL2 exists as a monomer or homodimer and binds to endothelial cells through proteoglycans. It interacts with TNFAIP6 via its Link domain. MCP-1/CCL2 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived MCP-1/CCL2 protein, expressed by HEK293 , with C-His labeled tag.
Background
MCP-1/CCL2 protein acts as a ligand for C-C chemokine receptor CCR2, leading to the activation and binding of CCR2 and subsequently inducing a strong chemotactic response and mobilization of intracellular calcium ions. It displays chemotactic activity specifically for monocytes and basophils, but not for neutrophils or eosinophils. Additionally, MCP-1/CCL2 plays a crucial role in mediating neuropathic pain resulting from peripheral nerve injury. It enhances NMDA-mediated synaptic transmission in dopamine D1 and D2 receptor-containing neurons, potentially through MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B. MCP-1/CCL2 exists as a monomer or homodimer, with its presence on endothelial cells facilitated by glycosaminoglycan (GAG) side chains of proteoglycans. Furthermore, it interacts with TNFAIP6 through its Link domain.
Technical Parameters
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Species Cynomolgus
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Source HEK293
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Tag C-8*His
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Accession
P61274 (Q24-P99)
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Molecular Construction
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N-term
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CCL2 (Q24-P99)
Accession # P61274 -
8*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
CCL2; Small Inducible Cytokine Subfamily A (Cys-Cys), Member 2; Prev. SCYA2; Chemokine (C-C Motif) Ligand 2; MCP-1; Monocyte Chemotactic Protein 1; MCP1; Small-Inducible Cytokine A2; MCAF; C-C Motif Chemokine 2; HC11; MGC9434; Monocyte Chemotactic And Act
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AA Sequence
QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQIQTPKP
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Predicted Molecular Mass
9.8 kDa
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Molecular Weight
Approximately 13-16 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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