MEK2 Protein, Human (Active, sf9, GST)
The MEK2 protein phosphorylates threonine and tyrosine residues in a Thr-Glu-Tyr sequence of MAP kinases. It activates ERK1 and ERK2 MAP kinases and BRAF through a KSR1 or KSR2-dependent mechanism. By binding to KSR1 or KSR2, it enables dimerization and activation of BRAF by releasing inhibitory intramolecular interactions (PubMed:29433126). MEK2 Protein, Human (sf9, GST) is the recombinant human-derived MEK2 protein, expressed by Sf9 insect cells , with N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The MEK2 protein phosphorylates threonine and tyrosine residues in a Thr-Glu-Tyr sequence of MAP kinases. It activates ERK1 and ERK2 MAP kinases and BRAF through a KSR1 or KSR2-dependent mechanism. By binding to KSR1 or KSR2, it enables dimerization and activation of BRAF by releasing inhibitory intramolecular interactions (PubMed:29433126). MEK2 Protein, Human (sf9, GST) is the recombinant human-derived MEK2 protein, expressed by Sf9 insect cells , with N-GST labeled tag.
Background
MEK2, a pivotal kinase in cellular signaling, catalyzes the simultaneous phosphorylation of a threonine and a tyrosine residue within the Thr-Glu-Tyr sequence of MAP kinases. This enzymatic activity results in the activation of ERK1 and ERK2 MAP kinases, contributing to the intricate network of cellular responses. MEK2's role extends to the activation of BRAF, a process regulated by its interaction with KSR1 or KSR2. Binding to KSR1 or KSR2 facilitates the release of inhibitory intramolecular interactions within KSR1 or KSR2, promoting the dimerization of KSR1 or KSR2 with BRAF and subsequent BRAF activation. This mechanism highlights MEK2's significance in orchestrating signaling cascades critical for cellular functions.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-GST
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Accession
P36507 (M1-V400)
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Molecular Construction
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N-term
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GST
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MEK2 (M1-V400)
Accession # P36507 -
C-term
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Protein Length
Full length
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Synonyms
MAP2K2; MAPK/ERK Kinase 2; Prev. PRKMK2; Mitogen-Activated Protein Kinase Kinase; MEK2; MAPKK 2; MKK2; MAPKK2; MAP Kinase Kinase 2; MEK 2; Dual Specificity Mitogen-Activated Protein Kinase Kinase 2; CFC4; ERK Activator Kinase 2; Mitogen-Activated Protein
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AA Sequence
MLARRKPVLPALTINPTIAEGPSPTSEGASEANLVDLQKKLEELELDEQQKKRLEAFLTQKAKVGELKDDDFERISELGAGNGGVVTKVQHRPSGLIMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKEAKRIPEEILGKVSIAVLRGLAYLREKHQIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMAPERLQGTHYSVQSDIWSMGLSLVELAVGRYPIPPPDAKELEAIFGRPVVDGEEGEPHSISPRPRPPGRPVSGHGMDSRPAMAIFELLDYIVNEPPPKLPNGVFTPDFQEFVNKCLIKNPAERADLKMLTNHTFIKRSEVEEVDFAGWLCKTLRLNQPGTPTRTAV
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Predicted Molecular Mass
71 kDa
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Molecular Weight
Approximately 70 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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