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  3. Melanoma antigen preferentially/PRAME, Human (His)

Melanoma antigen preferentially/PRAME, Human (His)

Cat. No.: HY-P72258
COA Handling Instructions

PRAME is located in the CRL2 E3 ubiquitin-protein ligase complex and directs ubiquitination and degradation, specifically targeting the truncated MSRB1/SEPX1 selenoprotein. It is recruited to active promoter regions and is involved in chromatin regulation. Melanoma antigen preferentially/PRAME, Human (His) is the recombinant human-derived Melanoma antigen preferentially/PRAME, expressed by E. coli , with N-6*His labeled tag. The total length of Melanoma antigen preferentially/PRAME, Human (His) is 509 a.a., with molecular weight of ~61.9 kDa.

For research use only. We do not sell to patients.

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Description

PRAME is located in the CRL2 E3 ubiquitin-protein ligase complex and directs ubiquitination and degradation, specifically targeting the truncated MSRB1/SEPX1 selenoprotein. It is recruited to active promoter regions and is involved in chromatin regulation. Melanoma antigen preferentially/PRAME, Human (His) is the recombinant human-derived Melanoma antigen preferentially/PRAME, expressed by E. coli , with N-6*His labeled tag. The total length of Melanoma antigen preferentially/PRAME, Human (His) is 509 a.a., with molecular weight of ~61.9 kDa.

Background

PRAME serves as the substrate-recognition component within the Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, orchestrating the ubiquitination and subsequent degradation of target proteins. The CRL2(PRAME) complex specifically mediates the ubiquitination and degradation of truncated MSRB1/SEPX1 selenoproteins generated by failed UGA/Sec decoding. In the nucleus, this complex is recruited to epigenetically and transcriptionally active promoter regions bound by nuclear transcription factor Y (NFY), suggesting its involvement in chromatin regulation. PRAME functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB, and RARG. This repression activity prevents retinoic acid-induced cell proliferation arrest, differentiation, and apoptosis. The multifaceted role of PRAME highlights its significance in protein modification, particularly in the context of protein ubiquitination and cellular regulatory processes.

Species

Human

Source

E. coli

Tag

N-6*His

Accession

P78395 (M1-N509)

Gene ID
Molecular Construction
N-term
6*His
PRAME (M1-N509)
Accession # P78395
C-term
Synonyms
CT130; MAPE; OIP4; OPA interacting protein 4
AA Sequence

MERRRLWGSIQSRYISMSVWTSPRRLVELAGQSLLKDEALAIAALELLPRELFPPLFMAAFDGRHSQTLKAMVQAWPFTCLPLGVLMKGQHLHLETFKAVLDGLDVLLAQEVRPRRWKLQVLDLRKNSHQDFWTVWSGNRASLYSFPEPEAAQPMTKKRKVDGLSTEAEQPFIPVEVLVDLFLKEGACDELFSYLIEKVKRKKNVLRLCCKKLKIFAMPMQDIKMILKMVQLDSIEDLEVTCTWKLPTLAKFSPYLGQMINLRRLLLSHIHASSYISPEKEEQYIAQFTSQFLSLQCLQALYVDSLFFLRGRLDQLLRHVMNPLETLSITNCRLSEGDVMHLSQSPSVSQLSVLSLSGVMLTDVSPEPLQALLERASATLQDLVFDECGITDDQLLALLPSLSHCSQLTTLSFYGNSISISALQSLLQHLIGLSNLTHVLYPVPLESYEDIHGTLHLERLAYLHARLRELLCELGRPSMVWLSANPCPHCGDRTFYDPEPILCPCFMPN

Molecular Weight

Approximately 61.9kDa

Purity
  • Greater than 90% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Melanoma antigen preferentially/PRAME, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Melanoma antigen preferentially/PRAME, Human (His)
Cat. No.:
HY-P72258
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