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Meprin alpha/MEP1A Protein, Human (HEK293, His)

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Based on 1 publication(s) in Google Scholar

Meprin alpha (MEP1A) protein functions as a metalloendopeptidase in the membrane. It is found in structures like the gut and metanephros. MEP1A primarily expresses in the adult kidney and large intestine, indicating potential tissue-specific functions. Meprin alpha/MEP1A Protein, Human (HEK293, His) is the recombinant human-derived Meprin alpha/MEP1A protein, expressed by HEK293 , with C-His labeled tag.

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  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Meprin alpha/MEP1A Protein, Human (HEK293, His)
  • Meprin alpha/MEP1A Protein, Human (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Meprin alpha (MEP1A) protein functions as a metalloendopeptidase in the membrane. It is found in structures like the gut and metanephros. MEP1A primarily expresses in the adult kidney and large intestine, indicating potential tissue-specific functions. Meprin alpha/MEP1A Protein, Human (HEK293, His) is the recombinant human-derived Meprin alpha/MEP1A protein, expressed by HEK293 , with C-His labeled tag.

Background

Meprin alpha (MEP1A) protein is predicted to exhibit metalloendopeptidase activity and is primarily located in the membrane. It is expressed in various structures, including the gut and metanephros. The expression of MEP1A is notably biased, with higher expression observed in the adult kidney and large intestine, suggesting potential roles in these tissues.

Verified Bioactivity

Measured by its ability to cleave 20 μM fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (HY-P2616A).The specific activity is 2404.48 pmol/min/μg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    Q16819/NP_005579.2 (V22-Q601)

  • Gene ID
    4224  [NCBI]
  • Molecular Construction
    • N-term
    • MEP1A (V22-Q601)
      Accession # Q16819/NP_005579.2
    • His
    • C-term
  • Protein Length

    Partial Extracellular Domain (with Propeptide)

  • Synonyms

    MEP1A; Meprin A, Alpha (PABA Peptide Hydrolase); Meprin A Subunit Alpha; PPH Alpha; PABA Peptide Hydrolase; BA268F1.1 (Meprin A Alpha (PABA Peptide Hydrolase)); Endopeptidase-2; Metalloendopeptidase; PPHA; Meprin A Subunit; N-Benzoyl-L-Tyrosyl-P-Amino-Ben

  • AA Sequence

    VPIKYLPEENVHDADFGEQKDISEINLAAGLDLFQGDILLQKSRNGLRDPNTRWTFPIPYILADNLGLNAKGAILYAFEMFRLKSCVDFKPYEGESSYIIFQQFDGCWSEVGDQHVGQNISIGQGCAYKAIIEHEILHALGFYHEQSRTDRDDYVNIWWDQILSGYQHNFDTYDDSLITDLNTPYDYESLMHYQPFSFNKNASVPTITAKIPEFNSIIGQRLDFSAIDLERLNRMYNCTTTHTLLDHCTFEKANICGMIQGTRDDTDWAHQDSAQAGEVDHTLLGQCTGAGYFMQFSTSSGSAEEAALLESRILYPKRKQQCLQFFYKMTGSPSDRLVVWVRRDDSTGNVRKLVKVQTFQGDDDHNWKIAHVVLKEEQKFRYLFQGTKGDPQNSTGGIYLDDITLTETPCPTGVWTVRNFSQVLENTSKGDKLQSPRFYNSEGYGFGVTLYPNSRESSGYLRLAFHVCSGENDAILEWPVENRQVIITILDQEPDVRNRMSSSMVFTTSKSHTSPAINDTVIWDRPSRVGTYHTDCNCFRSIDLGWSGFISHQMLKRRSFLKNDDLIIFVDFEDITHLSQ

  • Molecular Weight

    Approximately 80 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Meprin alpha/MEP1A Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Meprin alpha/MEP1A Protein, Human (HEK293, His)
  • Meprin alpha/MEP1A Protein, Human (HEK293, His) Product Image