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MERS-CoV Spike/S1 Protein (CHO, hFc)

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Based on 1 Customer Validation

MERS-CoV Spike/S1 Proteinas, a crucial component of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV), mediates viral entry into host cells by binding to the host receptor DPP4. The Spike/S1 protein initiates the infection process and is a key target for antiviral strategies. Understanding its structure and interactions is vital for developing effective interventions against MERS-CoV. MERS-CoV Spike/S1 Protein (CHO, hFc) is the recombinant Virus-derived MERS-CoV Spike/S1 protein, expressed by CHO , with C-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Virus
  • Source: CHO
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for MERS-CoV Spike/S1 Protein (CHO, hFc)
  • Experimental Validation Results for MERS-CoV Spike/S1 Protein (CHO, hFc)
  • MERS-CoV Spike/S1 Protein (CHO, hFc) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

MERS-CoV Spike/S1 Proteinas, a crucial component of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV), mediates viral entry into host cells by binding to the host receptor DPP4. The Spike/S1 protein initiates the infection process and is a key target for antiviral strategies. Understanding its structure and interactions is vital for developing effective interventions against MERS-CoV. MERS-CoV Spike/S1 Protein (CHO, hFc) is the recombinant Virus-derived MERS-CoV Spike/S1 protein, expressed by CHO , with C-hFc labeled tag.

Background

ABCD2, an ATP-dependent transporter belonging to the ATP-binding cassette (ABC) family, plays a crucial role in facilitating the transport of very long-chain fatty acid (VLCFA)-CoA from the cytosol to the peroxisome lumen. Similar to ABCD1, ABCD2 exhibits fatty acyl-CoA thioesterase (ACOT) and ATPase activities. According to the proposed model, VLCFA-CoA is transported in an ATP-dependent manner into peroxisomes after the hydrolysis of VLCFA-CoA, mediated by ABCD2's ACOT activity (probable). Notably, ABCD2 shares overlapping substrate specificities with ABCD1, particularly towards saturated fatty acids (FA) and monounsaturated FA (MUFA). However, ABCD2 demonstrates a distinct substrate preference for shorter VLCFA (C22:0) and polyunsaturated fatty acids (PUFA) such as C22:6-CoA and C24:6-CoA, as observed in vitro. This substrate specificity suggests a potential regulatory role for ABCD2 in very long-chain fatty acids (VLCFAs) and energy metabolism, particularly in the degradation and biosynthesis of fatty acids through beta-oxidation.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human DPPIV/CD26 (HY-P72975) is present at 5 μg/mL can bind Recombinant MERS-CoV Spike/S1. The ED50 for this effect is 0.4222 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When Recombinant Human DPPIV/CD26 (HY-P72975) is present at 5 μg/mL can bind Recombinant MERS-CoV Spike/S1. The ED50 for this effect is 0.4222 μg/mL.

Technical Parameters

  • Species Virus
  • Source CHO
  • Tag C-hFc
  • Accession

    K9N5Q8 (Y18-P747)

  • Gene ID
    1489668
  • Molecular Construction
    • N-term
    • MERS-CoV S1 (Y18-P747)
      Accession # K9N5Q8
    • hFc
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    Spike glycoprotein; S; S glycoprotein; E2; Peplomer protein; Spike Protein, S1 Subunit

  • AA Sequence

    YVDVGPDSVKSACIEVDIQQTFFDKTWPRPIDVSKADGIIYPQGRTYSNITITYQGLFPYQGDHGDMYVYSAGHATGTTPQKLFVANYSQDVKQFANGFVVRIGAAANSTGTVIISPSTSATIRKIYPAFMLGSSVGNFSDGKMGRFFNHTLVLLPDGCGTLLRAFYCILEPRSGNHCPAGNSYTSFATYHTPATDCSDGNYNRNASLNSFKEYFNLRNCTFMYTYNITEDEILEWFGITQTAQGVHLFSSRYVDLYGGNMFQFATLPVYDTIKYYSIIPHSIRSIQSDRKAWAAFYVYKLQPLTFLLDFSVDGYIRRAIDCGFNDLSQLHCSYESFDVESGVYSVSSFEAKPSGSVVEQAEGVECDFSPLLSGTPPQVYNFKRLVFTNCNYNLTKLLSLFSVNDFTCSQISPAAIASNCYSSLILDYFSYPLSMKSDLSVSSAGPISQFNYKQSFSNPTCLILATVPHNLTTITKPLKYSYINKCSRFLSDDRTEVPQLVNANQYSPCVSIVPSTVWEDGDYYRKQLSPLEGGGWLVASGSTVAMTEQLQMGFGITVQYGTDTNSVCPKLEFANDTKIASQLGNCVEYSLYGVSGRGVFQNCTAVGVRQQRFVYDAYQNLVGYYSDDGNYYCLRACVSVPVSVIYDKETKTHATLFGSVACEHISSTMSQYSRSTRSMLKRRDSTYGPLQTPVGCVLGLVNSSLFVEDCKLPLGQSLCALPDTPSTLTP

  • Molecular Weight

    Approximately 115-135 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant Human DPPIV/CD26 (HY-P72975) is present at 5 μg/mL can bind Recombinant MERS-CoV Spike/S1. The ED50 for this effect is 0.4222 μg/mL.

  • MERS-CoV Spike/S1 Protein (CHO, hFc) Product Image
    1/16
  • Experimental Validation Results for MERS-CoV Spike/S1 Protein (CHO, hFc)
  • Experimental Validation Results for MERS-CoV Spike/S1 Protein (CHO, hFc)
  • MERS-CoV Spike/S1 Protein (CHO, hFc) Product Image