2. Recombinant Proteins
  3. Receptor Proteins Biotinylated Proteins
  4. Cell Adhesion Molecules (CAMs)
  5. Mesothelin Protein, Human (HEK293, His-Avi)

Mesothelin Protein, Human (HEK293, His-Avi)

Cat. No.: HY-P78370
Handling Instructions

FITC-Labeled Mesothelin Protein, especially in its membrane-anchored forms, potentially mediates cellular adhesion, implicating its role in cell interactions. Furthermore, the associated Megakaryocyte-potentiating factor (MPF) actively enhances in vitro megakaryocyte colony formation, indicating its participation in fostering specialized blood cell colonies' development. Mesothelin Protein, Human (HEK293, His-Avi) is the recombinant human-derived Mesothelin protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of Mesothelin Protein, Human (HEK293, His-Avi) is 285 a.a., with molecular weight of 40-50 kDa.

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Description

FITC-Labeled Mesothelin Protein, especially in its membrane-anchored forms, potentially mediates cellular adhesion, implicating its role in cell interactions. Furthermore, the associated Megakaryocyte-potentiating factor (MPF) actively enhances in vitro megakaryocyte colony formation, indicating its participation in fostering specialized blood cell colonies' development. Mesothelin Protein, Human (HEK293, His-Avi) is the recombinant human-derived Mesothelin protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of Mesothelin Protein, Human (HEK293, His-Avi) is 285 a.a., with molecular weight of 40-50 kDa.
Background

FITC-Labeled Mesothelin Protein, particularly in its membrane-anchored forms, exhibits a potential role in cellular adhesion, suggesting involvement in mediating interactions between cells. Furthermore, the associated Megakaryocyte-potentiating factor (MPF) has been identified as a potent enhancer of megakaryocyte colony formation in vitro, indicating its active participation in fostering the development of these specialized blood cell colonies.
Species

Human
Source

HEK293
Tag

C-Avi;C-His
Accession

Q13421-2 (E296-G580)
Gene ID
Molecular Construction
N-term
Mesothelin (E296-G580)
Accession # Q13421-2
His-Avi
C-term
Synonyms
MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR
Molecular Weight

40-50 kDa
Purity

Greater than 95% as determined by Tris-Bis PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Mesothelin Protein, Human (HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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