MICB Protein, Human (286a.a, HEK293, His)
MICB Protein, Human (286a.a, HEK293, His) is the recombinant human-derived MICB protein, expressed by HEK293, with C-His tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
MICB Protein, Human (286a.a, HEK293, His) is the recombinant human-derived MICB protein, expressed by HEK293, with C-His tag.
Background
MICB encodes a heavily glycosylated protein that serves as a ligand for the NKG2D type II receptor. Upon binding, this ligand triggers the cytolytic response of various immune cells, including natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells expressing the receptor. Notably, MICB is stress-induced and shares similarities with MHC class I molecules, although it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing generates multiple transcript variants, adding to the complexity of its functional repertoire. The expression of MICB is broad, with notable presence in lymph nodes, bone marrow, and various other tissues, underscoring its role in immune responses across diverse physiological contexts.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
NP_005922.2 (A23-T308)
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Gene ID4277
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Molecular Construction
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N-term
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MICB (A23-T308)
Accession # NP_005922.2 -
His
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C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
MICB; MHC Class I Chain-Related Protein; MHC Class I Polypeptide-Related Sequence B; Stress Inducible Class I Homolog; PERB11.2; MHC Class I Mic-B Antigen; MHC Class I Antigen-Related Protein B; MICB Protein; MHC Class I Chain-Related Protein B; MICB Antigen; MHC Class I-Like Molecule PERB11.2-IMX; MIC-B; MHC Class I Chain-Related Molecule B
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AA Sequence
AEPHSLRYNLMVLSQDGSVQSGFLAEGHLDGQPFLRYDRQKRRAKPQGQWAENVLGAKTWDTETEDLTENGQDLRRTLTHIKDQKGGLHSLQEIRVCEIHEDSSTRGSRHFYYDGELFLSQNLETQESTVPQSSRAQTLAMNVTNFWKEDAMKTKTHYRAMQADCLQKLQRYLKSGVAIRRTVPPMVNVTCSEVSEGNITVTCRASSFYPRNITLTWRQDGVSLSHNTQQWGDVLPDGNGTYQTWVATRIRQGEEQRFTCYMEHSGNHGTHPVPSGKALVLQSQRT
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Predicted Molecular Mass
34.5 kDa
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Molecular Weight
Approximately 45-55 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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