MICB Protein, Human (286a.a, HEK293, His)

MICB Protein, Human (286a.a, HEK293, His) is the recombinant human-derived MICB protein, expressed by HEK293, with C-His tag.

MICB encodes a heavily glycosylated protein that serves as a ligand for the NKG2D type II receptor. Upon binding, this ligand triggers the cytolytic response of various immune cells, including natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells expressing the receptor. Notably, MICB is stress-induced and shares similarities with MHC class I molecules, although it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing generates multiple transcript variants, adding to the complexity of its functional repertoire. The expression of MICB is broad, with notable presence in lymph nodes, bone marrow, and various other tissues, underscoring its role in immune responses across diverse physiological contexts.

Human

HEK293

C-His

NP_005922.2 (A23-T308)

4277

AEPHSLRYNLMVLSQDGSVQSGFLAEGHLDGQPFLRYDRQKRRAKPQGQWAENVLGAKTWDTETEDLTENGQDLRRTLTHIKDQKGGLHSLQEIRVCEIHEDSSTRGSRHFYYDGELFLSQNLETQESTVPQSSRAQTLAMNVTNFWKEDAMKTKTHYRAMQADCLQKLQRYLKSGVAIRRTVPPMVNVTCSEVSEGNITVTCRASSFYPRNITLTWRQDGVSLSHNTQQWGDVLPDGNGTYQTWVATRIRQGEEQRFTCYMEHSGNHGTHPVPSGKALVLQSQRT

Approximately 45-55 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution of PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.