MME Protein, Mouse (HEK293, His)

MME Protein, with thermolysin-like specificity, crucially degrades opioid peptides like Met- and Leu-enkephalins, cleaving a Gly-Phe bond. It also catalyzes the cleavage of bradykinin, substance P, neurotensin peptides, angiotensin-1, angiotensin-2, and angiotensin 1-9. Involved in atrial natriuretic factor (ANF) degradation, MME exhibits UV-inducible elastase activity towards skin preelastic and elastic fibers. MME Protein, Mouse (HEK293, His) is the recombinant mouse-derived MME protein, expressed by HEK293 , with N-His labeled tag. The total length of MME Protein, Mouse (HEK293, His) is 699 a.a., with molecular weight (glycosylation form) of 82-110 kDa.

MME Protein exhibits a thermolysin-like specificity, primarily acting on polypeptides of up to 30 amino acids. It plays a crucial role in the degradation of opioid peptides, including Met- and Leu-enkephalins, by cleaving a Gly-Phe bond. Additionally, MME catalyzes the cleavage of bradykinin, substance P, and neurotensin peptides, as well as angiotensin-1, angiotensin-2, and angiotensin 1-9. It is involved in the degradation of the atrial natriuretic factor (ANF) and demonstrates UV-inducible elastase activity towards skin preelastic and elastic fibers.

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK (Dnp) -OH. The specific activity is >3000 pmoles/min/μg.

Mouse

HEK293

N-His

Q61391/NP_032630.2 (Y52-W750)

17380  [NCBI]

YDDGICKSSDCIKSAARLIQNMDASVEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLKLLPDIYGWPVASDNWDQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDKNSTQHIIHFDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQSLPIDENQLSLEMNKVMELEKEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEVNGKSFSWSNFTNEIMSTVNINIQNEEEVVVYAPEYLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNENKLNNEYLELNYREDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSYMNPERKCRVW

82-110 kDa (glycosylation)

Greater than 95% as determined by Tris-Bis PAGE

Lyophilized powder

Lyophilized from 0.2μm filtered solution in PBS (pH 7.4) or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.