MRCKβ Protein, Human (sf9, GST)
CDC42BPB protein is a key serine/threonine protein kinase downstream of CDC42 that controls cytoskeletal dynamics and cell migration. CDC42BPB phosphorylates PPP1R12C and MYL9/MLC2, regulates actin reorganization and shapes cell dynamics. MRCKβ Protein, Human (sf9, GST) is the recombinant human-derived MRCKβ, expressed by Sf9 insect cells, with GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
CDC42BPB protein is a key serine/threonine protein kinase downstream of CDC42 that controls cytoskeletal dynamics and cell migration. CDC42BPB phosphorylates PPP1R12C and MYL9/MLC2, regulates actin reorganization and shapes cell dynamics. MRCKβ Protein, Human (sf9, GST) is the recombinant human-derived MRCKβ, expressed by Sf9 insect cells, with GST labeled tag.
Background
CDC42BPB Protein, a serine/threonine-protein kinase, stands as a pivotal downstream effector of CDC42, exerting significant influence over cytoskeleton reorganization and cell migration. Through the phosphorylation of PPP1R12C and MYL9/MLC2, CDC42BPB orchestrates the regulation of actin cytoskeletal reorganization, emphasizing its role in shaping cellular dynamics. Collaborating with MYO18A and LURAP1, CDC42BPB actively participates in the modulation of lamellar actomyosin retrograde flow, a process critical for cell protrusion and migration. Furthermore, CDC42BPB phosphorylates PPP1R12A, contributing to its multifaceted impact on cellular functions. In tandem with FAM89B/LRAP25, CDC42BPB facilitates the targeting of LIMK1 to the lamellipodium, initiating its activation and subsequent phosphorylation of CFL1, a crucial event for the regulation of lamellipodial F-actin dynamics.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag GST
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Accession
Q9Y5S2 (S2-G473)
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Gene ID9578
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Protein Length
Partial
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Synonyms
CDC42BPB; CDC42-Binding Protein Kinase Beta; CDC42 Binding Protein Kinase Beta; CDC42BP-Beta; Myotonic Dystrophy Kinase-Related CDC42-Binding Kinase Beta; MRCK Beta; Serine/Threonine-Protein Kinase MRCK Beta; CDC42-Binding Protein Kinase Beta (DMPK-Like);
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AA Sequence
SAKVRLKKLEQLLLDGPWRNESALSVETLLDVLVCLYTECSHSALRRDKYVAEFLEWAKPFTQLVKEMQLHREDFEIIKVIGRGAFGEVAVVKMKNTERIYAMKILNKWEMLKRAETACFREERDVLVNGDCQWITALHYAFQDENHLYLVMDYYVGGDLLTLLSKFEDKLPEDMARFYIGEMVLAIDSIHQLHYVHRDIKPDNVLLDVNGHIRLADFGSCLKMNDDGTVQSSVAVGTPDYISPEILQAMEDGMGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHEERFQFPSHVTDVSEEAKDLIQRLICSRERRLGQNGIEDFKKHAFFEGLNWENIRNLEAPYIPDVSSPSDTSNFDVDDDVLRNTEILPPGSHTGFSGLHLPFIGFTFTTESCFSDRGSLKSIMQSNTLTKDEDVQRDLEHSLQMEAYERRIRRLEQEKLELSRKLQESTQTVQSLHG
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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