Nectin-1 Protein, Rat (HEK293, His)

Nectin-1 Protein, with carbohydrate and virion binding activities, plays pivotal roles in axon guidance, synapse assembly, and viral entry. Located in dendrites, growth cone membranes, and synapses, Nectin-1 is implicated in human conditions like cleft lip and ectodermal dysplasia. Its biased expression, especially in Kidney (RPKM 45.4) and Brain (RPKM 31.3), underscores its significance in diverse physiological contexts. Nectin-1 Protein, Rat (HEK293, His) is the recombinant rat-derived Nectin-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Nectin-1 Protein, Rat (HEK293, His) is 324 a.a., with molecular weight of 55-60 kDa.

Nectin-1 Protein, characterized by its carbohydrate binding and virion binding activities, plays pivotal roles in axon guidance, regulation of synapse assembly, and viral entry into host cells. Distributed across various cellular components, including dendrites, growth cone membranes, and synapses, Nectin-1 is implicated in human conditions such as cleft lip, cleft lip-palate-ectodermal dysplasia syndrome, cleft palate, and ectodermal dysplasia. The biased expression pattern, notably in the Kidney (RPKM 45.4), Brain (RPKM 31.3), and nine other tissues, underscores its significance in diverse physiological contexts.

Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 is present at 1 μg/mL, can bind Recombinant Rat Nectin-1. The ED₅₀ for this effect is 125.7 ng/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 is present at 1 μg/mL, can bind Recombinant Rat Nectin-1.The ED₅₀ for this effect is 125.7 ng/mL.

Rat

HEK293

C-His

NP_001093946 (Q31-A354)

192183  [NCBI]

QVVQVNDSMYGFIGTDVILHCSFANPLPTVKITQVTWQKASNGSKQNMAIYNPTMGVSVLPPYEKRVEFLRPSFIDGTIRLSHLELEDEGMYICEFATFPTGNRESQLNLTVMAKPTNWIEGTQAVLRARKGQDDKVLVATCTSANGKPPSVVSWETRLKGEAEYQEIRNPNGTVTVISRYRLVPSREAHRQSLACIVNYHLDRFRESLTLNVQYEPEVTIEGFDGNWYLQRTDVKLTCKADANPPATEYHWTTLNGSLPKGVEAQNRTLFFRGPINYSLAGTYICEATNPIGTRSGQVEVNITEFPYTPTPEHGRRAGQMPTA

Approximately 50-70 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.