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Nectin-1 Protein, Rat (HEK293, His)

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Based on 1 Customer Validation

Nectin-1 Protein, with carbohydrate and virion binding activities, plays pivotal roles in axon guidance, synapse assembly, and viral entry. Located in dendrites, growth cone membranes, and synapses, Nectin-1 is implicated in human conditions like cleft lip and ectodermal dysplasia. Its biased expression, especially in Kidney (RPKM 45.4) and Brain (RPKM 31.3), underscores its significance in diverse physiological contexts. Nectin-1 Protein, Rat (HEK293, His) is the recombinant rat-derived Nectin-1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Nectin-1 Protein, Rat (HEK293, His)
  • Experimental Validation Results for Nectin-1 Protein, Rat (HEK293, His)
  • Nectin-1 Protein, Rat (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Nectin-1 Protein, with carbohydrate and virion binding activities, plays pivotal roles in axon guidance, synapse assembly, and viral entry. Located in dendrites, growth cone membranes, and synapses, Nectin-1 is implicated in human conditions like cleft lip and ectodermal dysplasia. Its biased expression, especially in Kidney (RPKM 45.4) and Brain (RPKM 31.3), underscores its significance in diverse physiological contexts. Nectin-1 Protein, Rat (HEK293, His) is the recombinant rat-derived Nectin-1 protein, expressed by HEK293 , with C-His labeled tag.

Background

Nectin-1 Protein, characterized by its carbohydrate binding and virion binding activities, plays pivotal roles in axon guidance, regulation of synapse assembly, and viral entry into host cells. Distributed across various cellular components, including dendrites, growth cone membranes, and synapses, Nectin-1 is implicated in human conditions such as cleft lip, cleft lip-palate-ectodermal dysplasia syndrome, cleft palate, and ectodermal dysplasia. The biased expression pattern, notably in the Kidney (RPKM 45.4), Brain (RPKM 31.3), and nine other tissues, underscores its significance in diverse physiological contexts.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 is present at 1 μg/mL, can bind Recombinant Rat Nectin-1. The ED50 for this effect is 125.7 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 is present at 1 μg/mL, can bind Recombinant Rat Nectin-1.The ED50 for this effect is 125.7 ng/mL.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-His
  • Accession

    F1LNP8/NP_001093946 (Q31-A354)

  • Gene ID
    192183  [NCBI]
  • Molecular Construction
    • N-term
    • Nectin-1 (Q31-A354)
      Accession # F1LNP8/NP_001093946
    • His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    NECTIN1; OFC7; Prev. PVRL1; Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C); Prev. HVEC; Herpesvirus Ig-Like Receptor; Prev. ED4; Ectodermal Dysplasia 4 (Margarita Island Type); Nectin-1; Herpes Simplex Virus Type 1 Sensitivity; PRR1; Herpesv

  • AA Sequence

    QVVQVNDSMYGFIGTDVILHCSFANPLPTVKITQVTWQKASNGSKQNMAIYNPTMGVSVLPPYEKRVEFLRPSFIDGTIRLSHLELEDEGMYICEFATFPTGNRESQLNLTVMAKPTNWIEGTQAVLRARKGQDDKVLVATCTSANGKPPSVVSWETRLKGEAEYQEIRNPNGTVTVISRYRLVPSREAHRQSLACIVNYHLDRFRESLTLNVQYEPEVTIEGFDGNWYLQRTDVKLTCKADANPPATEYHWTTLNGSLPKGVEAQNRTLFFRGPINYSLAGTYICEATNPIGTRSGQVEVNITEFPYTPTPEHGRRAGQMPTA

  • Molecular Weight

    Approximately 55-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 is present at 1 μg/mL, can bind Recombinant Rat Nectin-1.The ED50 for this effect is 125.7 ng/mL.

  • Nectin-1 Protein, Rat (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Nectin-1 Protein, Rat (HEK293, His)
  • Experimental Validation Results for Nectin-1 Protein, Rat (HEK293, His)
  • Nectin-1 Protein, Rat (HEK293, His) Product Image