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MME/Neprilysin Protein, Rat (HEK293, His)

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Based on 1 Customer Validation

Neprilysin/CD10 protein has a specificity similar to thermolysin and mainly acts on polypeptides of up to 30 amino acids. It plays a crucial role in degrading opioid peptides, including Met-enkephalin and Leu-enkephalin, by cleaving the Gly-Phe bond. MME/Neprilysin Protein, Rat (HEK293, His) is the recombinant rat-derived Neprilysin/CD10 protein, expressed by HEK293 , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for MME/Neprilysin Protein, Rat (HEK293, His)
  • MME/Neprilysin Protein, Rat (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Neprilysin/CD10 protein has a specificity similar to thermolysin and mainly acts on polypeptides of up to 30 amino acids. It plays a crucial role in degrading opioid peptides, including Met-enkephalin and Leu-enkephalin, by cleaving the Gly-Phe bond. MME/Neprilysin Protein, Rat (HEK293, His) is the recombinant rat-derived Neprilysin/CD10 protein, expressed by HEK293 , with N-His labeled tag.

Background

The Neprilysin/CD10 Protein exhibits a thermolysin-like specificity but is primarily active on polypeptides of up to 30 amino acids. Biologically significant, it plays a crucial role in the degradation of opioid peptides, including Met- and Leu-enkephalins, achieved through the cleavage of a Gly-Phe bond. Additionally, Neprilysin/CD10 catalyzes the cleavage of bradykinin, substance P, and neurotensin peptides, and it can cleave angiotensin-1, angiotensin-2, and angiotensin 1-9. The protein is implicated in the degradation of the atrial natriuretic factor (ANF) and exhibits UV-inducible elastase activity towards skin preelastic and elastic fibers, highlighting its diverse enzymatic functions (By similarity).

Verified Bioactivity

Measured by its ability to cleave a peptide substrate, Mca-(ala7,lys(dnp)9)-bradykinin. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read). The specific activity is 12527.20 pmoL/min/µg, as measured under the described conditions.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag N-His
  • Accession

    P07861 (Y52-W750)

  • Gene ID
    24590  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • Neprilysin (Y52-W750)
      Accession # P07861
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    MME; Neprilysin-390; Membrane Metalloendopeptidase; Neprilysin-411; Neprilysin; SFE; CALLA; Membrane Metallo-Endopeptidase (Neutral Endopeptidase, Enkephalinase, CALLA, CD10); NEP; Membrane Metallo-Endopeptidase Variant 2; Neutral Endopeptidase 24.11; Alt

  • AA Sequence

    YDDGICKSSDCIKSAARLIQNMDASAEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLTLLPDIYGWPVASQNWEQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDKNSTQHIIHFDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQRLPIDENQLSLEMNKVMELEKEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEINGKPFSWSNFTNEIMSTVNINIQNEEEVVVYAPEYLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNENKLNNEYLELNYKEEEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSARQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFTWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSYMNPERKCRVW

  • Molecular Weight

    Approximately 93 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • MME/Neprilysin Protein, Rat (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for MME/Neprilysin Protein, Rat (HEK293, His)
  • MME/Neprilysin Protein, Rat (HEK293, His) Product Image