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NES Protein, Human (P.pastoris, His)

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Based on 1 publication(s) in Google Scholar

NES is critical for brain and eye development and promotes the disassembly of phosphorylated vimentin intermediate filaments during mitosis. It directs the transport of IF proteins and other factors to daughter cells during progenitor cell division, promoting the survival, renewal, and proliferation of neural progenitor cells. NES Protein, Human (P.pastoris, His) is the recombinant human-derived NES protein, expressed by P. pastoris , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: P. pastoris
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

NES is critical for brain and eye development and promotes the disassembly of phosphorylated vimentin intermediate filaments during mitosis. It directs the transport of IF proteins and other factors to daughter cells during progenitor cell division, promoting the survival, renewal, and proliferation of neural progenitor cells. NES Protein, Human (P.pastoris, His) is the recombinant human-derived NES protein, expressed by P. pastoris , with N-6*His labeled tag.

Background

NES is essential for brain and eye development, playing a crucial role in promoting the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis. This protein is implicated in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division, contributing to the survival, renewal, and mitogen-stimulated proliferation of neural progenitor cells. In vitro, NES forms homodimers and homotetramers, with a propensity to preferentially form heterodimers with other intermediate filament proteins such as vimentin and alpha-internexin. Notably, the assembly of these heterodimers into intermediate filaments occurs if nestin levels do not exceed 25%. Additionally, NES interacts with FHOD3, further highlighting its involvement in intricate cellular processes (By similarity).

Technical Parameters

  • Species Human
  • Source P. pastoris
  • Tag N-6*His
  • Accession

    P48681 (P1321-L1569)

  • Gene ID
    10763  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • NES (P1321-L1569)
      Accession # P48681
    • C-term
  • Protein Length

    Partial

  • Synonyms

    NES; NES Protein; Nestin; Nbla00170; FLJ21841

  • AA Sequence

    PPPQGETGKEGWDPAVLASEGLEAPPSEKEEGEEGEEECGRDSDLSEEFEDLGTEAPFLPGVPGEVAEPLGQVPQLLLDPAAWDRDGESDGFADEEESGEEGEEDQEEGREPGAGRWGPGSSVGSLQALSSSQRGEFLESDSVSVSVPWDDSLRGAVAGAPKTALETESQDSAEPSGSEEESDPVSLEREDKVPGPLEIPSGMEDAGPGADIIGVNGQGPNLEGKSQHVNGGVMNGLEQSEEVGQGMPL

  • Predicted Molecular Mass

    27.8 kDa

  • Molecular Weight

    Approximately 38 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HC1, 0.5 M NaCl, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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