NES Protein, Human (P.pastoris, His)

NES is critical for brain and eye development and promotes the disassembly of phosphorylated vimentin intermediate filaments during mitosis. It directs the transport of IF proteins and other factors to daughter cells during progenitor cell division, promoting the survival, renewal, and proliferation of neural progenitor cells. NES Protein, Human (P.pastoris, His) is the recombinant human-derived NES protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of NES Protein, Human (P.pastoris, His) is 249 a.a., with molecular weight of ~38 kDa.

NES is essential for brain and eye development, playing a crucial role in promoting the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis. This protein is implicated in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division, contributing to the survival, renewal, and mitogen-stimulated proliferation of neural progenitor cells. In vitro, NES forms homodimers and homotetramers, with a propensity to preferentially form heterodimers with other intermediate filament proteins such as vimentin and alpha-internexin. Notably, the assembly of these heterodimers into intermediate filaments occurs if nestin levels do not exceed 25%. Additionally, NES interacts with FHOD3, further highlighting its involvement in intricate cellular processes (By similarity).

Human

P. pastoris

N-6*His

P48681 (P1321-L1569)

10763  [NCBI]

PPPQGETGKEGWDPAVLASEGLEAPPSEKEEGEEGEEECGRDSDLSEEFEDLGTEAPFLPGVPGEVAEPLGQVPQLLLDPAAWDRDGESDGFADEEESGEEGEEDQEEGREPGAGRWGPGSSVGSLQALSSSQRGEFLESDSVSVSVPWDDSLRGAVAGAPKTALETESQDSAEPSGSEEESDPVSLEREDKVPGPLEIPSGMEDAGPGADIIGVNGQGPNLEGKSQHVNGGVMNGLEQSEEVGQGMPL

Approximately 38 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.