Neurolysin Protein, Human (His)
Based on 1 Customer Validation
Neurolysin protein is a multifunctional enzyme that hydrolyzes oligopeptides such as neurotensin, bradykinin, and dynorphin A. In addition to its role in oligopeptide metabolism, Neurolysin also modulates cannabinoid signaling pathways by promoting the degradation of vasopressin, an antagonist peptide of the cannabinoid receptor CNR1. Neurolysin Protein, Human (His) is the recombinant human-derived Neurolysin protein, expressed by E. coli , with N-His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Neurolysin protein is a multifunctional enzyme that hydrolyzes oligopeptides such as neurotensin, bradykinin, and dynorphin A. In addition to its role in oligopeptide metabolism, Neurolysin also modulates cannabinoid signaling pathways by promoting the degradation of vasopressin, an antagonist peptide of the cannabinoid receptor CNR1. Neurolysin Protein, Human (His) is the recombinant human-derived Neurolysin protein, expressed by E. coli , with N-His labeled tag.
Background
Neurolysin protein serves as a versatile enzyme with the ability to hydrolyze various oligopeptides, including neurotensin, bradykinin, and dynorphin A. Beyond its role in oligopeptide metabolism, Neurolysin assumes a regulatory function in the cannabinoid signaling pathway. It accomplishes this by facilitating the degradation of hemopressin, an antagonist peptide that interacts with the cannabinoid receptor CNR1. This dual role positions Neurolysin as a key player in both the enzymatic breakdown of biologically active peptides and the modulation of cannabinoid receptor signaling through the regulation of hemopressin levels.
Verified Bioactivity
Measured by its ability to cleave a fluorogenic peptide substrate (7-methoxycoumarin-4-yl) acetyl Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl)-OH and the specific activity is > 100 pmol/min/μg.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-His
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Accession
Q9BYT8 (S38-P704)
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Molecular Construction
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N-term
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His
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Neurolysin (S38-P704)
Accession # Q9BYT8 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
NLN; Neurolysin (Metallopeptidase M3 Family); Prev. AGTBP; MEP; Neurotensin Endopeptidase; Angiotensin-Binding Protein; Mitochondrial Oligopeptidase M; Endopeptidase 24.16; Neurolysin, Mitochondrial; EP24.16; Microsomal Endopeptidase; MOP; KIAA1226; Neuro
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AA Sequence
SSYTVAGRNVLRWDLSPEQIKTRTEELIVQTKQVYDAVGMLGIEEVTYENCLQALADVEVKYIVERTMLDFPQHVSSDKEVRAASTEADKRLSRFDIEMSMRGDIFERIVHLQETCDLGKIKPEARRYLEKSIKMGKRNGLHLPEQVQNEIKSMKKRMSELCIDFNKNLNEDDTFLVFSKAELGALPDDFIDSLEKTDDDKYKITLKYPHYFPVMKKCCIPETRRRMEMAFNTRCKEENTIILQQLLPLRTKVAKLLGYSTHADFVLEMNTAKSTSRVTAFLDDLSQKLKPLGEAEREFILNLKKKECKDRGFEYDGKINAWDLYYYMTQTEELKYSIDQEFLKEYFPIEVVTEGLLNTYQELLGLSFEQMTDAHVWNKSVTLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMAVAALVVNFSQPVAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDVDSLRRLSKHYKDGSPIADDLLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNTSLDAASEYAKYCSEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFYSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDMLHNFLKREPNQKAFLMSRGLHAP
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Predicted Molecular Mass
78.7 kDa
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Molecular Weight
Approximately 67 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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