Neurolysin Protein, Human (His)

Neurolysin protein is a multifunctional enzyme that hydrolyzes oligopeptides such as neurotensin, bradykinin, and dynorphin A. In addition to its role in oligopeptide metabolism, Neurolysin also modulates cannabinoid signaling pathways by promoting the degradation of vasopressin, an antagonist peptide of the cannabinoid receptor CNR1. Neurolysin Protein, Human (His) is the recombinant human-derived Neurolysin protein, expressed by E. coli , with N-His labeled tag. The total length of Neurolysin Protein, Human (His) is 667 a.a., with molecular weight of ~67 kDa.

Neurolysin protein serves as a versatile enzyme with the ability to hydrolyze various oligopeptides, including neurotensin, bradykinin, and dynorphin A. Beyond its role in oligopeptide metabolism, Neurolysin assumes a regulatory function in the cannabinoid signaling pathway. It accomplishes this by facilitating the degradation of hemopressin, an antagonist peptide that interacts with the cannabinoid receptor CNR1. This dual role positions Neurolysin as a key player in both the enzymatic breakdown of biologically active peptides and the modulation of cannabinoid receptor signaling through the regulation of hemopressin levels.

Measured by its ability to cleave a fluorogenic peptide substrate (7-methoxycoumarin-4-yl) acetyl Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl)-OH and the specific activity is > 100 pmol/min/μg.

Human

E. coli

N-His

Q9BYT8 (S38-P704)

57486  [NCBI]

SSYTVAGRNVLRWDLSPEQIKTRTEELIVQTKQVYDAVGMLGIEEVTYENCLQALADVEVKYIVERTMLDFPQHVSSDKEVRAASTEADKRLSRFDIEMSMRGDIFERIVHLQETCDLGKIKPEARRYLEKSIKMGKRNGLHLPEQVQNEIKSMKKRMSELCIDFNKNLNEDDTFLVFSKAELGALPDDFIDSLEKTDDDKYKITLKYPHYFPVMKKCCIPETRRRMEMAFNTRCKEENTIILQQLLPLRTKVAKLLGYSTHADFVLEMNTAKSTSRVTAFLDDLSQKLKPLGEAEREFILNLKKKECKDRGFEYDGKINAWDLYYYMTQTEELKYSIDQEFLKEYFPIEVVTEGLLNTYQELLGLSFEQMTDAHVWNKSVTLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMAVAALVVNFSQPVAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDVDSLRRLSKHYKDGSPIADDLLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNTSLDAASEYAKYCSEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFYSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDMLHNFLKREPNQKAFLMSRGLHAP

Approximately 67 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.