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Neuroplastin Protein, Human (HEK293, His)

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Neuroplastin protein is a possible cell adhesion molecule that plays a crucial role in the long-term potentiation of hippocampal synapses and regulates neurite growth through the p38MAPK and FGFR1 pathways.It acts as a chaperone of ATP2B1, stabilizing and enhancing its ATPase activity, affecting cellular calcium homeostasis.Neuroplastin Protein, Human (HEK293, His) is the recombinant human-derived Neuroplastin protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Neuroplastin protein is a possible cell adhesion molecule that plays a crucial role in the long-term potentiation of hippocampal synapses and regulates neurite growth through the p38MAPK and FGFR1 pathways.It acts as a chaperone of ATP2B1, stabilizing and enhancing its ATPase activity, affecting cellular calcium homeostasis.Neuroplastin Protein, Human (HEK293, His) is the recombinant human-derived Neuroplastin protein, expressed by HEK293 , with C-6*His labeled tag.

Background

The Neuroplastin protein is recognized as a probable homophilic and heterophilic cell adhesion molecule, playing a crucial role in long-term potentiation at hippocampal excitatory synapses through the activation of p38MAPK. Additionally, it is implicated in the regulation of neurite outgrowth by activating the FGFR1 signaling pathway and may contribute to synaptic plasticity. Notably, Neuroplastin acts as a chaperone for ATP2B1, stabilizing ATP2B1 and enhancing its ATPase activity, thereby influencing cellular calcium homeostasis. Furthermore, Neuroplastin promotes the localization of XKR8 at the cell membrane, indicating its involvement in membrane dynamics and cellular processes. The intricate interactions of Neuroplastin with ATP2B1 and XKR8 underline its multifunctional roles in both cell adhesion and the modulation of key cellular pathways.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q9Y639-1 (Q29-H220)

  • Gene ID
    27020  [NCBI]
  • Molecular Construction
    • N-term
    • Neuroplastin (Q29-H220)
      Accession # Q9Y639-1
    • 6*His
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    NPTN; GP65; Prev. SDFR1; Np55; SDR1; Stromal Cell Derived Factor Receptor 1; Stromal Cell-Derived Receptor 1; SDR-1; GP55; DKFZp566H1924; Neuroplastin; Np65

  • AA Sequence

    QNEPRIVTSEEVIIRDSPVLPVTLQCNLTSSSHTLTYSYWTKNGVELSATRKNASNMEYRINKPRAEDSGEYHCVYHFVSAPKANATIEVKAAPDITGHKRSENKNEGQDATMYCKSVGYPHPDWIWRKKENGMPMDIVNTSGRFFIINKENYTELNIVNLQITEDPGEYECNATNAIGSASVVTVLRVRSH

  • Predicted Molecular Mass

    22.7 kDa

  • Molecular Weight

    Approximately 35-55 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
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ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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