Neuroplastin Protein, Human (192a.a, HEK293, His)

Neuroplastin protein is a possible cell adhesion molecule that plays a crucial role in the long-term potentiation of hippocampal synapses and regulates neurite growth through the p38MAPK and FGFR1 pathways. It acts as a chaperone of ATP2B1, stabilizing and enhancing its ATPase activity, affecting cellular calcium homeostasis. Neuroplastin Protein, Human (192a.a, HEK293, His) is the recombinant human-derived Neuroplastin protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Neuroplastin Protein, Human (192a.a, HEK293, His) is 192 a.a., with molecular weight of ~40.0 kDa.

The Neuroplastin protein is recognized as a probable homophilic and heterophilic cell adhesion molecule, playing a crucial role in long-term potentiation at hippocampal excitatory synapses through the activation of p38MAPK. Additionally, it is implicated in the regulation of neurite outgrowth by activating the FGFR1 signaling pathway and may contribute to synaptic plasticity. Notably, Neuroplastin acts as a chaperone for ATP2B1, stabilizing ATP2B1 and enhancing its ATPase activity, thereby influencing cellular calcium homeostasis. Furthermore, Neuroplastin promotes the localization of XKR8 at the cell membrane, indicating its involvement in membrane dynamics and cellular processes. The intricate interactions of Neuroplastin with ATP2B1 and XKR8 underline its multifunctional roles in both cell adhesion and the modulation of key cellular pathways.

Human

HEK293

C-6*His

Q9Y639 (Q29-H220)

27020  [NCBI]

QNEPRIVTSEEVIIRDSPVLPVTLQCNLTSSSHTLTYSYWTKNGVELSATRKNASNMEYRINKPRAEDSGEYHCVYHFVSAPKANATIEVKAAPDITGHKRSENKNEGQDATMYCKSVGYPHPDWIWRKKENGMPMDIVNTSGRFFIINKENYTELNIVNLQITEDPGEYECNATNAIGSASVVTVLRVRSH

Approximately 40.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.