NOV/CCN3 Protein, Human (sf9, His)

NOV/CCN3 proteins perform a wide range of functions, including proliferation, adhesion, migration, differentiation, and survival. It interacts with various proteins, such as integrins and membrane receptors, affecting different cellular responses. NOV/CCN3 Protein, Human (sf9, His) is the recombinant human-derived NOV/CCN3 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of NOV/CCN3 Protein, Human (sf9, His) is 326 a.a., with molecular weight of approximately 47 kDa.

NOV/CCN3 protein, an immediate-early participant in cellular processes, orchestrates a wide array of functions, including proliferation, adhesion, migration, differentiation, and survival. This multifaceted role is underscored by its interactions with integrins and membrane receptors, such as NOTCH1, influencing diverse cellular responses. As an essential regulator of hematopoietic stem and progenitor cell function, NOV/CCN3 inhibits myogenic differentiation through the activation of the Notch-signaling pathway. It also exerts inhibitory effects on vascular smooth muscle cells proliferation independently of TGFB1 signaling, showcasing its regulatory influence on cell-cycle regulators. Functioning as a ligand for integrins ITGAV:ITGB3 and ITGA5:ITGB1, NOV/CCN3 directly stimulates pro-angiogenic activities, induces angiogenesis, and promotes endothelial cell adhesion, migration, and survival. Additionally, it plays a role in cutaneous wound healing, supports skin fibroblast adhesion and chemotaxis, and enhances bFGF-induced DNA synthesis in fibroblasts. In bone regeneration, NOV/CCN3 acts as a negative regulator, influencing the articular chondrocytic phenotype while repressing endochondral ossification. It also impacts pancreatic beta-cell function by inhibiting proliferation and insulin secretion. Notably, NOV/CCN3 acts as a negative regulator of endothelial pro-inflammatory activation, attenuates inflammatory pain, and interacts with various proteins, including FBLN1, NOTCH1, GJA1/CX43, ITGA5:ITGB1, ITGAV:ITGB3, ITGAV:ITGB5, and ZDHHC22. These intricate interactions highlight NOV/CCN3's central role in orchestrating diverse cellular functions and regulatory pathways.

Human

Sf9 insect cells

C-His

P48745/NP_002505.1 (T32-M357)

4856

approximately 47 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 50mM Tris, 100mM NaCl, 0.5mM PMSF, 10mM Imidazole, 10% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.