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NRG1-beta 1 Protein, Human (246a.a, His)

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Based on 1 Customer Validation

NRG1 is a direct ligand of the ERBB3 and ERBB4 tyrosine kinase receptors. NRG1 can induce the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1. NRG1 can induce cell growth, differentiation and proliferation. NRG1-beta 1 Protein, Human (246a.a, His) is the recombinant human-derived NRG1-beta 1 protein, expressed by E. coli , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for NRG1-beta 1 Protein, Human (246a.a, His)
  • Experimental Validation Results for NRG1-beta 1 Protein, Human (246a.a, His)
  • NRG1-beta 1 Protein, Human (246a.a, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

NRG1 is a direct ligand of the ERBB3 and ERBB4 tyrosine kinase receptors. NRG1 can induce the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1. NRG1 can induce cell growth, differentiation and proliferation. NRG1-beta 1 Protein, Human (246a.a, His) is the recombinant human-derived NRG1-beta 1 protein, expressed by E. coli , with N-6*His labeled tag.

Background

Pro-neuregulin-1, membrane-bound isoform (NRG1) is a direct ligand of the ERBB3 and ERBB4 tyrosine kinase receptors. Several isomers of NRG1 can induce the growth and differentiation of epithelial cells, glial cells, neuronal cells and skeletal muscle cells. The induced expression of acetylcholine receptors in synaptic vesicles during the formation of neuromuscular junction stimulates the germination and lactation of breast lobular alveoli and induces the differentiation of breast tumor cells. Stimulate Schwann cell proliferation; It affects the development of the heart muscle. NRG1 is also a ligand for integrins and binds (via the EGF domain) the integrins ITGAV: ITGB3 or ITGA6:ITGB4. NRG1 can induce the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1[1][2][3][4].

Verified Bioactivity

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 33.21 ng/mL, corresponding to a specific activity is 3.01×104 units/mg

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 33.21 ng/mL, corresponding to a specific activity is 3.01×104 units/mg.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His
  • Accession

    AAA58639.1 (M1-K246)

  • Gene ID
    3084  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • NRG1-beta 1 (M1-K246)
      Accession # AAA58639.1
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    NRG1; NRG1 Class VII Isoform Alpha 2a; Prev. HGL; NRG1 Class VII Isoform Beta 2a; Prev. NRG1-IT2; Neuregulin 1 Isoform HRG-Gamma; NDF; Neuregulin 1 Isoform HRG-Beta3; GGF; Neuregulin 1 Isoform HRG-Beta2; Pro-Neuregulin-1, Membrane-Bound Isoform; Neureguli

  • AA Sequence

    MSERKEGRGKGKGKKKERGSGKKPESAAGSQSPALPPQLKEMKSQESAAGSKLVLRCETSSEYSSLRFKWFKNGNELNRKNKPQNIKIQKKPGKSELRINKASLADSGEYMCKVISKLGNDSASANITIVESNEIITGMPASTEGAYVSSESPIRISVSTEGANTSSSTSTSTTGTSHLVKCAEKEKTFCVNGGECFMVKDLSNPSRYLCKCPNEFTGDRCQNYVMASFYKHLGIEFMEAEELYQK

  • Molecular Weight

    Approximately 35 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of 4 mM HCl or 50 mM Tris-HCl, 300 mM NaCl, 200 mM arginine, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 33.21 ng/mL, corresponding to a specific activity is 3.01×104 units/mg.

  • NRG1-beta 1 Protein, Human (246a.a, His) Product Image
    1/16
  • Experimental Validation Results for NRG1-beta 1 Protein, Human (246a.a, His)
  • Experimental Validation Results for NRG1-beta 1 Protein, Human (246a.a, His)
  • NRG1-beta 1 Protein, Human (246a.a, His) Product Image