OPN4 Protein, Rat (Cell-Free, His)
OPN4 Protein, a photoreceptor with affinity for cis-retinaldehydes, crucially mediates light responses like the pupillary reflex and photoentrainment. Its multifaceted role includes potential participation in optokinetic visual tracking response. Moreover, OPN4 may regulate retinal hyaloid vessel growth, emphasizing its significance in intricate aspects of ocular physiology. OPN4 Protein, Rat (Cell-Free, His) is the recombinant rat-derived OPN4 protein, expressed by E. coli Cell-free, with N-10*His labeled tag.
- Species: Rat
- Source: E. coli Cell-free
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Biological Activity
Description
OPN4 Protein, a photoreceptor with affinity for cis-retinaldehydes, crucially mediates light responses like the pupillary reflex and photoentrainment. Its multifaceted role includes potential participation in optokinetic visual tracking response. Moreover, OPN4 may regulate retinal hyaloid vessel growth, emphasizing its significance in intricate aspects of ocular physiology. OPN4 Protein, Rat (Cell-Free, His) is the recombinant rat-derived OPN4 protein, expressed by E. coli Cell-free, with N-10*His labeled tag.
Background
OPN4, a photoreceptor with an affinity for cis-retinaldehydes, plays a pivotal role in various light-mediated responses, including the pupillary reflex, photoentrainment, and other non-image forming reactions. Its involvement extends to potential participation in the optokinetic visual tracking response, showcasing its multifaceted role in visual processes. Additionally, OPN4 may contribute to the regulation of retinal hyaloid vessel growth and regression, further emphasizing its significance in orchestrating intricate aspects of ocular physiology.
Technical Parameters
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Species Rat
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Source E. coli Cell-free
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Tag N-10*His
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Accession
Q8R456 (M1-M474)
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Gene ID192223
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Molecular Construction
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N-term
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10*His
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OPN4 (M1-M474)
Accession # Q8R456 -
C-term
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Protein Length
Full Length
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Synonyms
Melanopsin; Opsin-4; Opn4
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AA Sequence
MNSPSESRVPSSLTQDPSFTASPALLQGIWNSTQNISVRVQLLSVSPTTPGLQAAAWVPFPTVDVPDHAHYTLGTVILLVGLTGMLGNLTVIYTFCRNRGLRTPANMLIINLAVSDFLMSFTQAPVFFASSLYKKWLFGETGCKFYAFCGAVFGIVSMITLTAIAMDRYLVITRPLATIGMRSKRRTALVLLGVWLYALAWSLPPFFGWSAYVPEGLLTSCSWDYVTFTPLVRAYTMLLFCFVFFLPLLIIIFCYIFIFRAIRETGRACEGCGESPLRRRQWQRLQSEWKMAKVALIVILLFVLSWAPYSTVALVGFAGYSHILTPYMSSVPAVIAKASAIHNPIIYAITHPKYRAAIAQHLPCLGVLLGVSGQRSHPSLSYRSTHRSTLSSQSSDLSWISGQKRQESLGSESEVGWTDTETTAAWGAAQQASGQSFCSHDLEDGEVKAPSSPQEQKSKTPKTKRHLPSLDRRM
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Predicted Molecular Mass
53.9 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)