OTUD7B Protein, Human (sf9)

The OTUD7B protein acts as a negative regulator to inhibit the non-canonical NF-kappa-B pathway by deubiquitinating TRAF3, thereby inhibiting B cell responses. It prevents degradation of “Lys-48”-linked polyubiquitin chains on TRAF3 during atypical NF-kappa-B stimulation, avoiding overactivation of the pathway and affecting mucosal immunity. OTUD7B Protein, Human (sf9) is the recombinant human-derived OTUD7B protein, expressed by sf9 insect cells , with tag free.

OTUD7B protein acts as a negative regulator of the non-canonical NF-kappa-B pathway by facilitating the deubiquitination of TRAF3, a suppressor of NF-kappa-B signaling, thereby dampening B-cell responses. In the context of non-canonical NF-kappa-B stimuli, OTUD7B targets 'Lys-48'-linked polyubiquitin chains on TRAF3, preventing its proteolysis and averting excessive activation of the non-canonical NF-kappa-B pathway. This negative regulatory role extends to mucosal immunity against infections. Additionally, OTUD7B modulates T cell receptor (TCR) signaling by deubiquitinating ZAP70, influencing NF-kappa-B activation in T cells. Furthermore, OTUD7B contributes to T cell homeostasis, playing a crucial role in normal T cell responses and the production of key cytokines such as IFNG and IL2. The protein also exhibits deubiquitinating activity towards EGFR and demonstrates versatility in hydrolyzing various polyubiquitin chain linkages, including 'Lys-11', 'Lys-48', and 'Lys-63'-linked polyubiquitin chains. While it exhibits a higher catalytic rate with 'Lys-11'-linked chains in vitro, the physiological significance of this preference remains uncertain.

The fundamental role of OTUD7B is specific removal of ubiquitin from substrates. OTUD7B catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission light under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader.

Human

Sf9 insect cells

Tag Free

Q6GQQ9 (T2-F843)

56957

Partial

TLDMDAVLSDFVRSTGAEPGLARDLLEGKNWDVNAALSDFEQLRQVHAGNLPPSFSEGSGGSRTPEKGFSDREPTRPPRPILQRQDDIVQEKRLSRGISHASSSIVSLARSHVSSNGGGGGSNEHPLEMPICAFQLPDLTVYNEDFRSFIERDLIEQSMLVALEQAGRLNWWVSVDPTSQRLLPLATTGDGNCLLHAASLGMWGFHDRDLMLRKALYALMEKGVEKEALKRRWRWQQTQQNKESGLVYTEDEWQKEWNELIKLASSEPRMHLGTNGANCGGVESSEEPVYESLEEFHVFVLAHVLRRPIVVVADTMLRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYDQAHFSALVSMEQKENTKEQAVIPLTDSEYKLLPLHFAVDPGKGWEWGKDDSDNVRLASVILSLEVKLHLLHSYMNVKWIPLSSDAQAPLAQPESPTASAGDEPRSTPESGDSDKESVGSSSTSNEGGRRKEKSKRDREKDKKRADSVANKLGSFGKTLGSKLKKNMGGLMHSKGSKPGGVGTGLGGSSGTETLEKKKKNSLKSWKGGKEEAAGDGPVSEKPPAESVGNGGSKYSQEVMQSLSILRTAMQGEGKFIFVGTLKMGHRHQYQEEMIQRYLSDAEERFLAEQKQKEAERKIMNGGIGGGPPPAKKPEPDAREEQPTGPPAESRAMAFSTGYPGDFTIPRPSGGGVHCQEPRRQLAGGPCVGGLPPYATFPRQCPPGRPYPHQDSIPSLEPGSHSKDGLHRGALLPPPYRVADSYSNGYREPPEPDGWAGGLRGLPPTQTKCKQPNCSFYGHPETNNFCSCCYREELRRREREPDGELLVHRF

92.5 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 20% glycerol, pH 7.5, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.