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p63/TP73L Protein, Human (sf9, His-GST)

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The p63/TP73L protein is a sequence-specific DNA-binding transcriptional regulator that exhibits diverse activities through its isoforms. Isoform 2 activates RIPK4 transcription, and binding of this protein to TP73/p73 initiates p53/TP53-dependent apoptosis in response to genotoxic stress. p63/TP73L Protein, Human (sf9, His-GST) is the recombinant human-derived p63/TP73L protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The p63/TP73L protein is a sequence-specific DNA-binding transcriptional regulator that exhibits diverse activities through its isoforms. Isoform 2 activates RIPK4 transcription, and binding of this protein to TP73/p73 initiates p53/TP53-dependent apoptosis in response to genotoxic stress. p63/TP73L Protein, Human (sf9, His-GST) is the recombinant human-derived p63/TP73L protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

Background

p63/TP73L Protein functions as a sequence-specific DNA binding transcriptional regulator with diverse activities mediated by its isoforms, each containing distinct transactivation and auto-regulating transactivation inhibiting domains. Isoform 2 specifically activates RIPK4 transcription, while the protein's engagement with TP73/p73 is crucial for initiating p53/TP53-dependent apoptosis in response to genotoxic stress and activated oncogenes. Additionally, p63 contributes to Notch signaling, potentially inducing JAG1 and JAG2, and plays a role in epithelial morphogenesis. The balance between DeltaN-type and TA*-type isoforms likely governs the maintenance of epithelial stem cell compartments and influences the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Furthermore, the protein is indispensable for limb formation from the apical ectodermal ridge and exerts its regulatory role by activating the transcription of the p21 promoter.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-His;N-GST
  • Accession

    Q9H3D4 (M1-E680)

  • Gene ID
    8626  [NCBI]
  • Molecular Construction
    • N-term
    • His-GST
    • p63 (M1-E680)
      Accession # Q9H3D4
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    TP63; Chronic Ulcerative Stomatitis Protein; Prev. TP73L; Tumor Protein P53-Competing Protein; Prev. TP53CP; Transformation-Related Protein 63; Prev. TP53L; Tumor Protein P73-Like; P73L; Tumor Protein 63; P73H; Amplified In Squamous Cell Carcinoma; P51; C

  • AA Sequence

    MNFETSRCATLQYCPDPYIQRFVETPAHFSWKESYYRSTMSQSTQTNEFLSPEVFQHIWDFLEQPICSVQPIDLNFVDEPSEDGATNKIEISMDCIRMQDSDLSDPMWPQYTNLGLLNSMDQQIQNGSSSTSPYNTDHAQNSVTAPSPYAQPSSTFDALSPSPAIPSNTDYPGPHSFDVSFQQSSTAKSATWTYSTELKKLYCQIAKTCPIQIKVMTPPPQGAVIRAMPVYKKAEHVTEVVKRCPNHELSREFNEGQIAPPSHLIRVEGNSHAQYVEDPITGRQSVLVPYEPPQVGTEFTTVLYNFMCNSSCVGGMNRRPILIIVTLETRDGQVLGRRCFEARICACPGRDRKADEDSIRKQQVSDSTKNGDGTKRPFRQNTHGIQMTSIKKRRSPDDELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQHQHLLQKQTSIQSPSSYGNSSPPLNKMNSMNKLPSVSQLINPQQRNALTPTTIPDGMGANIPMMGTHMPMAGDMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCSIVSFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQLHEFSSPSHLLRTPSSASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDARRNKQQRIKEEGE

  • Predicted Molecular Mass

    105 kDa

  • Molecular Weight

    Approximately 125 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris,300mM NaCl, pH7.5, 10%glycerol, 0.5mM TCEP, 0.5mM PMSF, 0.1mM EDTA, 0.5 mM GSH.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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