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PADI4 Protein, Human (Biotinylated, His)

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The PADI4 protein catalyzes the citrullination of arginine residues in proteins such as histones, contributing to the histone code and stem cell regulation. PADI4 Protein, Human (Biotinylated, His) is the recombinant human-derived PADI4 protein, expressed by E. coli , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The PADI4 protein catalyzes the citrullination of arginine residues in proteins such as histones, contributing to the histone code and stem cell regulation. PADI4 Protein, Human (Biotinylated, His) is the recombinant human-derived PADI4 protein, expressed by E. coli , with N-His labeled tag.

Background

PADI4, a protein crucial for the histone code, catalyzes the citrullination/deimination of arginine residues in various proteins, including histones. Specifically, it citrullinates histone H1 at 'Arg-54' (forming H1R54ci), histone H3 at 'Arg-2,' 'Arg-8,' 'Arg-17,' and/or 'Arg-26' (forming H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively), and histone H4 at 'Arg-3' (forming H4R3ci). This enzymatic activity plays a key role in the regulation of stem cell maintenance by inducing chromatin decondensation and pluripotency through the displacement of H1 from chromatin. Additionally, PADI4 is involved in the innate immune response, mediating chromatin decondensation in neutrophils during infection and contributing to the formation of neutrophil extracellular traps (NETs). Furthermore, it prevents the methylation of histone H3 by CARM1 and HRMT1L2/PRMT1, thereby repressing transcription. Notably, PADI4 citrullinates EP300/P300 at 'Arg-2142,' promoting its interaction with NCOA2/GRIP1.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-10*His
  • Accession

    Q9UM07/AAH25718.1 (M1-P663)

  • Gene ID
    23569  [NCBI]
  • Molecular Construction
    • N-term
    • 10*His
    • PADI4 (M1-P663)
      Accession # Q9UM07/AAH25718.1
    • C-term
  • Protein Length

    Full Length

  • Conjugation

    Biotin

  • Synonyms

    PADI4; Protein-Arginine Deiminase Type IV; Prev. PADI5; HL-60 PAD; PDI5; PAD4; PDI4; Peptidyl Arginine Deiminase Type IV; PAD; Peptidylarginine Deiminase IV; Peptidyl Arginine Deiminase, Type IV; PADI-H Protein; Peptidyl Arginine Deiminase, Type V; PADI4

  • AA Sequence

    MAQGTLIRVTPEQPTHAVCVLGTLTQLDICSSAPEDCTSFSINASPGVVVDIAHGPPAKKKSTGSSTWPLDPGVEVTLTMKVASGSTGDQKVQISYYGPKTPPVKALLYLTGVEISLCADITRTGKVKPTRAVKDQRTWTWGPCGQGAILLVNCDRDNLESSAMDCEDDEVLDSEDLQDMSLMTLSTKTPKDFFTNHTLVLHVARSEMDKVRVFQATRGKLSSKCSVVLGPKWPSHYLMVPGGKHNMDFYVEALAFPDTDFPGLITLTISLLDTSNLELPEAVVFQDSVVFRVAPWIMTPNTQPPQEVYACSIFENEDFLKSVTTLAMKAKCKLTICPEEENMDDQWMQDEMEIGYIQAPHKTLPVVFDSPRNRGLKEFPIKRVMGPDFGYVTRGPQTGGISGLDSFGNLEVSPPVTVRGKEYPLGRILFGDSCYPSNDSRQMHQALQDFLSAQQVQAPVKLYSDWLSVGHVDEFLSFVPAPDRKGFRLLLASPRSCYKLFQEQQNEGHGEALLFEGIKKKKQQKIKNILSNKTLREHNSFVERCIDWNRELLKRELGLAESDIIDIPQLFKLKEFSKAEAFFPNMVNMLVLGKHLGIPKPFGPVINGRCCLEEKVCSLLEPLGLQCTFINDFFTYHIRHGEVHCGTNVRRKPFSFKWWNMVP

  • Predicted Molecular Mass

    76.6 kDa

  • Molecular Weight

    Approximately 77 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 0.5 mM TCEP, 1 mM EDTA, 10% glycerol, pH 7.6.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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