Pentraxin 2/SAP Protein, Rat (HEK293, His)
Based on 1 Customer Validation
Pentraxin 2/SAP Protein forms a discoid arrangement of five non-covalently bound subunits as a homopentamer. Pentraxin 2/SAP Protein, Rat (HEK293, His) is the recombinant rat-derived Pentraxin 2/SAP protein, expressed by HEK293 , with C-His labeled tag.
- Species: Rat
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Pentraxin 2/SAP Protein forms a discoid arrangement of five non-covalently bound subunits as a homopentamer. Pentraxin 2/SAP Protein, Rat (HEK293, His) is the recombinant rat-derived Pentraxin 2/SAP protein, expressed by HEK293 , with C-His labeled tag.
Background
Pentraxin 2/SAP Protein is a homopentamer that exhibits a discoid arrangement of five non-covalently bound subunits.
Verified Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Rat Pentraxin 2 at 2 μg/mL (100 μL/well) on the plate can bind Biotinylated Human CD32a (HY-P70711). The ED50 for this effect is 2.336 μg/mL.
MCE Validation Data
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Bioactivity - ELISA
Bioactivity - ELISA
Technical Parameters
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Species Rat
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Source HEK293
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Tag C-His
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Accession
P23680 (Q21-S228)
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Gene ID29339 [NCBI]
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Molecular Construction
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N-term
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SAP (Q21-S228)
Accession # P23680 -
His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
APCS; Pentraxin-2; Amyloid P Component, Serum; MGC88159; PTX2; Epididymis Secretory Sperm Binding Protein Li 92n; SAP; Pentraxin Family Member; 9.5S Alpha-1-Glycoprotein; Pentaxin-Related; Serum Amyloid P-Component; HEL-S-92n; Pentraxin-Related
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AA Sequence
QTDLNQKVFVFPRESETDYVKLIPWLEKPLQNFTLCFRAYSDLSRSQSLFSYSVNSRDNELLIYKDKVGQYSLYIGNSKVTVRGLEEFPSPIHFCTSWESSSGIAEFWVNGKPWVKKGLQKGYTVKSSPSIVLGQEQDTYGGGFDKTQSFVGEIADLYMWDSVLTPENIHSVDRGFPPNPNILDWRALNYEINGYVVIKPRMWDNKSS
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Molecular Weight
Approximately 30 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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