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PH20/SPAM1 Protein, Human (Active, HEK293, His)

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PH20/SPAM1 facilitates sperm-egg adhesion during fertilization. It plays a crucial role in penetrating the cumulus cell layer surrounding the egg, which is embedded in a hyaluronic acid matrix formed before ovulation. This protein achieves penetration by digesting hyaluronic acid. PH20/SPAM1 Protein, Human (HEK293, His) is the recombinant human-derived PH20/SPAM1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • PH20/SPAM1 Protein, Human (Active, HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

PH20/SPAM1 facilitates sperm-egg adhesion during fertilization. It plays a crucial role in penetrating the cumulus cell layer surrounding the egg, which is embedded in a hyaluronic acid matrix formed before ovulation. This protein achieves penetration by digesting hyaluronic acid. PH20/SPAM1 Protein, Human (HEK293, His) is the recombinant human-derived PH20/SPAM1 protein, expressed by HEK293 , with C-His labeled tag.

Background

PH20/SPAM1 protein plays a crucial role in sperm-egg adhesion during fertilization. In the intricate process of fertilization, sperm encounters a layer of cumulus cells surrounding the egg before reaching the zona pellucida. These cumulus cells are embedded in a matrix rich in hyaluronic acid, formed in preparation for ovulation. PH20/SPAM1 facilitates sperm penetration through the cumulus cell layer by digesting hyaluronic acid. By engaging in this enzymatic activity, PH20/SPAM1 contributes to the essential steps of fertilization, enabling the sperm to navigate through the cumulus cell barrier and reach the zona pellucida for successful fertilization to occur.

Verified Bioactivity

The activity of Human Hyaluronidase PH-20 Protein, His Tag is measured by its ability to hydrolyze HA in turbidimetric assay (45 minute assay). The specific activity is >60,000 U/mg. (Unit Definition: One unit of Hyaluronidase activity will cause a change in A600 of 0.330 per minute at pH 5.35 at 37°C in a 2.0 mL reaction mixture)

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    P38567-1 (L36-Y482)

  • Gene ID
    6677  [NCBI]
  • Molecular Construction
    • N-term
    • PH20 (L36-Y482)
      Accession # P38567-1
    • His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    SPAM1; Hyaluronidase PH-20; Sperm Adhesion Molecule 1; HEL-S-96n; SPAG15; Hyal-PH20; HYAL5; PH20; PH-20; Epididymis Secretory Sperm Binding Protein Li 96n; Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding); Hyaluronidase; Hyaluronogl

  • AA Sequence

    LNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFY

  • Predicted Molecular Mass

    53 kDa

  • Molecular Weight

    Approximately 60-66 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 7.5. Normally 10% trehalose is added as protectant before lyophilization.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • PH20/SPAM1 Protein, Human (Active, HEK293, His) Product Image
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  • PH20/SPAM1 Protein, Human (Active, HEK293, His) Product Image