PH20/SPAM1 Protein, Human (Active, HEK293, His)

PH20/SPAM1 facilitates sperm-egg adhesion during fertilization. It plays a crucial role in penetrating the cumulus cell layer surrounding the egg, which is embedded in a hyaluronic acid matrix formed before ovulation. This protein achieves penetration by digesting hyaluronic acid. PH20/SPAM1 Protein, Human (HEK293, His) is the recombinant human-derived PH20/SPAM1 protein, expressed by HEK293 , with C-His labeled tag.

PH20/SPAM1 protein plays a crucial role in sperm-egg adhesion during fertilization. In the intricate process of fertilization, sperm encounters a layer of cumulus cells surrounding the egg before reaching the zona pellucida. These cumulus cells are embedded in a matrix rich in hyaluronic acid, formed in preparation for ovulation. PH20/SPAM1 facilitates sperm penetration through the cumulus cell layer by digesting hyaluronic acid. By engaging in this enzymatic activity, PH20/SPAM1 contributes to the essential steps of fertilization, enabling the sperm to navigate through the cumulus cell barrier and reach the zona pellucida for successful fertilization to occur.

The activity of Human Hyaluronidase PH-20 Protein, His Tag is measured by its ability to hydrolyze HA in turbidimetric assay (45 minute assay). The specific activity is >60,000 U/mg. (Unit Definition: One unit of Hyaluronidase activity will cause a change in A600 of 0.330 per minute at pH 5.35 at 37°C in a 2.0 mL reaction mixture)

Human

HEK293

C-His

P38567-1 (L36-Y482)

6677  [NCBI]

Partial

LNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFY

Approximately 60-66 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 7.5. Normally 10% trehalose is added as protectant before lyophilization.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.