PI4KB Protein, Human (Active, sf9, His, GST)
The PI4KB protein is a key enzyme that initiates the production of 1,4,5-trisphosphate inositol (PIP) by phosphorylating phosphatidylinositol (PI). In addition to myo-inositol signaling, it is involved in mitotic Golgi disassembly and Golgi-to-plasma membrane trafficking, which is critical for cell membrane dynamics. PI4KB Protein, Human (sf9, His, GST) is the recombinant human-derived PI4KB protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The PI4KB protein is a key enzyme that initiates the production of 1,4,5-trisphosphate inositol (PIP) by phosphorylating phosphatidylinositol (PI). In addition to myo-inositol signaling, it is involved in mitotic Golgi disassembly and Golgi-to-plasma membrane trafficking, which is critical for cell membrane dynamics. PI4KB Protein, Human (sf9, His, GST) is the recombinant human-derived PI4KB protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.
Background
PI4KB, a pivotal enzyme, phosphorylates phosphatidylinositol (PI), initiating the crucial step in generating the second messenger inositol-1,4,5-trisphosphate (PIP). Beyond its fundamental role in inositol signaling, PI4KB is implicated in Golgi disintegration/reorganization during mitosis, possibly through its phosphorylation. Additionally, it participates in Golgi-to-plasma membrane trafficking, underscoring its significance in cellular membrane dynamics. Furthermore, PI4KB emerges as a key player in inner ear development, suggesting diverse regulatory functions beyond its role in membrane trafficking. In the context of microbial infection, PI4KB assumes a critical role in Aichi virus RNA replication, being recruited by ACBD3 at the viral replication sites. This multifaceted involvement highlights PI4KB's versatile contributions to cellular processes and viral replication mechanisms.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-8*His;N-GST
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Accession
Q9UBF8 (G2-M816)
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Gene ID5298
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Molecular Construction
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N-term
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8*His-GST
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PI4KB (G2-M816)
Accession # Q9UBF8 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
PI4KB; Phosphatidylinositol 4-Kinase, Wortmannin-Sensitive; Prev. PIK4CB; PI4KIIIBETA; PI4K-BETA; PI4K-Beta; Pi4K92; PI4KBETA; Phosphatidylinositol 4-Kinase, Catalytic, Beta; PI4Kbeta; PtdIns 4-Kinase Beta; DFNA87; PI4KIII; PI4K92; NPIK; Phosphatidylinosi
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AA Sequence
GDTVVEPAPLKPTSEPTSGPPGNNGGSLLSVITEGVGELSVIDPEVAQKACQEVLEKVKLLHGGVAVSSRGTPLELVNGDGVDSEIRCLDDPPAQIREEEDEMGAAVASGTAKGARRRRQNNSAKQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFCFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVHRCRQSINFSLQCALLLGAYSSDMHISTQRHSRGTKLRKLILSDELKPAHRKRELPSLSPAPDTGLSPSKRTHQRSKSDATASISLSSNLKRTASNPKVENEDEELSSSTESIDNSFSSPVRLAPEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQLAHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKKQSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSPRNLGFETSAFKLTTEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHGSSTIRNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGIM
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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