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PICALM Protein, Human (HEK293, His)

PICALM Protein, Human (HEK293, His) plays a critical role in recruiting clathrin and adapter protein complex 2 (AP2) to the cell membrane during coated pit formation and clathrin-vesicle assembly effect. It regulates the size of the clathrin cage and determines membrane recycling. PICALM Protein, Human (HEK293, His) is the recombinant human-derived PICALM protein, expressed by HEK293 , with C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

PICALM Protein, Human (HEK293, His) plays a critical role in recruiting clathrin and adapter protein complex 2 (AP2) to the cell membrane during coated pit formation and clathrin-vesicle assembly effect. It regulates the size of the clathrin cage and determines membrane recycling. PICALM Protein, Human (HEK293, His) is the recombinant human-derived PICALM protein, expressed by HEK293 , with C-10*His labeled tag.

Background

The PICALM gene encodes a clathrin assembly protein crucial for recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes during coated-pit formation and clathrin-vesicle assembly. Its role may extend to regulating the size of the clathrin cage, thus determining the amount of membrane to be recycled. This protein is implicated in AP2-dependent clathrin-mediated endocytosis, particularly at the neuromuscular junction. Notably, chromosomal translocation involving this gene is associated with various hematological malignancies. Additionally, polymorphisms in this gene are linked to Alzheimer's disease risk. The gene exhibits ubiquitous expression, with notable levels observed in fat, appendix, and 25 other tissues. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    Q13492/NP_009097 (M1-M652)

  • Gene ID
    8301
  • Molecular Construction
    • N-term
    • PICALM (M1-M652)
      Accession # NP_009097
    • 10*His
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    PICALM; CLTH; Phosphatidylinositol Binding Clathrin Assembly Protein; Clathrin Assembly Lymphoid Myeloid Leukemia Protein; CALM; Alternative Protein PICALM; Phosphatidylinositol-Binding Clathrin Assembly Protein; LAP

  • AA Sequence

    MSGQSLTDRITAAQHSVTGSAVSKTVCKATTHEIMGPKKKHLDYLIQCTNEMNVNIPQLADSLFERTTNSSWVVVFKSLITTHHLMVYGNERFIQYLASRNTLFNLSNFLDKSGLQGYDMSTFIRRYSRYLNEKAVSYRQVAFDFTKVKRGADGVMRTMNTEKLLKTVPIIQNQMDALLDFNVNSNELTNGVINAAFMLLFKDAIRLFAAYNEGIINLLEKYFDMKKNQCKEGLDIYKKFLTRMTRISEFLKVAEQVGIDRGDIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQARLKALKEQRLKELAKKPHTSLTTAASPVSTSAGGIMTAPAIDIFSTPSSSNSTSKLPNDLLDLQQPTFHPSVHPMSTASQVASTWGDPFSATVDAVDDAIPSLNPFLTKSSGDVHLSISSDVSTFTTRTPTHEMFVGFTPSPVAQPHPSAGLNVDFESVFGNKSTNVIVDSGGFDELGGLLKPTVASQNQNLPVAKLPPSKLVSDDLDSSLANLVGNLGIGNGTTKNDVNWSQPGEKKLTGGSNWQPKVAPTTAWNAATMAPPVMAYPATTPTGMIGYGIPPQMGSVPVMTQPTLIYSQPVMRPPNPFGPVSGAQIQFM

  • Predicted Molecular Mass

    72.1 kDa

  • Molecular Weight

    Approximately 90-150 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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