PKLR Protein, Human (HEK293, His)

The PKLR protein is a key enzyme in cellular metabolism that acts as a pyruvate kinase, converting phosphoenolpyruvate to pyruvate. This process is an integral part of glycolysis, the fundamental pathway for cellular energy production. PKLR Protein, Human (HEK293, His) is the recombinant human-derived PKLR protein, expressed by HEK293 , with C-6*His labeled tag.

PKLR, a crucial enzyme in cellular metabolism, functions as a pyruvate kinase that facilitates the conversion of phosphoenolpyruvate to pyruvate. This enzymatic process is integral to glycolysis, a fundamental pathway in cellular energy production. Through its catalytic activity, PKLR contributes to the synthesis of ATP, a vital energy currency in the cell. The enzyme's role in glycolysis underscores its significance in the regulation of energy metabolism and highlights its essential function in supporting various cellular processes dependent on ATP availability.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P30613-1 (M1-S574)

5313  [NCBI]

Full Length of Isoform-1

MSIQENISSLQLRSWVSKSQRDLAKSILIGAPGGPAGYLRRASVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAIALDTKGPEIRTGILQGGPESEVELVKGSQVLVTVDPAFRTRGNANTVWVDYPNIVRVVPVGGRIYIDDGLISLVVQKIGPEGLVTQVENGGVLGSRKGVNLPGAQVDLPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS

Approximately 62.0 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 500 mM NaCl, 5% trehalose, 5% mannitol, 0.02% Tween 80, 50% glycerol, 1 mM EDTA, 1 mM DTT, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.