Plexin B2 Protein, Human (HEK293, His)

Plexin B2 is a cell surface receptor for SEMA4C, SEMA4D, and SEMA4G and is involved in glutamatergic synapse development and SEMA4A-mediated excitatory synapse development. Plexin B2 activates downstream RHOA and ERBB2 phosphorylation, which is critical for promoting neuronal differentiation and migration. Plexin B2 Protein, Human (HEK293, His) is the recombinant human-derived Plexin B2 protein, expressed by HEK293 , with C-His labeled tag.

Plexin B2 (PLXNB2) serves as a cell surface receptor for SEMA4C, SEMA4D, and SEMA4G, playing a crucial role in cell-cell signaling. It is involved in glutamatergic synapse development and is required for SEMA4A-mediated excitatory synapse development. Binding to class 4 semaphorins induces downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248.' PLXNB2 is essential for normal differentiation and migration of neuronal cells during brain corticogenesis, contributing to normal embryonic brain development, and regulating the migration of cerebellar granule cells in the developing brain. Additionally, it plays a pivotal role in RHOA activation, leading to changes in the actin cytoskeleton, and participates in axon guidance, invasive growth, and cell migration. PLXNB2 may modulate the activity of RAC1 and CDC42, existing as both a monomer and a heterodimer with PLXNB1. Furthermore, it interacts with SEMA4C, SEMA4D, SEMA4G, MET, ARHGEF11, ARHGEF12, and possibly MST1R.

Immobilized Human Plexin B2 His at 5 μg/mL (100 μL/well) can bind Human SEMA4D Fc with a linear range of 0.039-1.25 μg/mL.

Human

HEK293

C-His

O15031-1 (L20-R1160)

23654  [NCBI]

Partial

LRPRKLDFFRSEKELNHLAVDEASGVVYLGAVNALYQLDAKLQLEQQVATGPALDNKKCTPPIEASQCHEAEMTDNVNQLLLLDPPRKRLVECGSLFKGICALRALSNISLRLFYEDGSGEKSFVASNDEGVATVGLVSSTGPGGDRVLFVGKGNGPHDNGIIVSTRLLDRTDSREAFEAYTDHATYKAGYLSTNTQQFVAAFEDGPYVFFVFNQQDKHPARNRTLLARMCREDPNYYSYLEMDLQCRDPDIHAAAFGTCLAASVAAPGSGRVLYAVFSRDSRSSGGPGAGLCLFPLDKVHAKMEANRNACYTGTREARDIFYKPFHGDIQCGGHAPGSSKSFPCGSEHLPYPLGSRDGLRGTAVLQRGGLNLTAVTVAAENNHTVAFLGTSDGRILKVYLTPDGTSSEYDSILVEINKRVKRDLVLSGDLGSLYAMTQDKVFRLPVQECLSYPTCTQCRDSQDPYCGWCVVEGRCTRKAECPRAEEASHWLWSRSKSCVAVTSAQPQNMSRRAQGEVQLTVSPLPALSEEDELLCLFGESPPHPARVEGEAVICNSPSSIPVTPPGQDHVAVTIQLLLRRGNIFLTSYQYPFYDCRQAMSLEENLPCISCVSNRWTCQWDLRYHECREASPNPEDGIVRAHMEDSCPQFLGPSPLVIPMNHETDVNFQGKNLDTVKGSSLHVGSDLLKFMEPVTMQESGTFAFRTPKLSHDANETLPLHLYVKSYGKNIDSKLHVTLYNCSFGRSDCSLCRAANPDYRCAWCGGQSRCVYEALCNTTSECPPPVITRIQPETGPLGGGIRITILGSNLGVQAGDIQRISVAGRNCSFQPERYSVSTRIVCVIEAAETPFTGGVEVDVFGKLGRSPPNVQFTFQQPKPLSVEPQQGPQAGGTTLTIHGTHLDTGSQEDVRVTLNGVPCKVTKFGAQLQCVTGPQATRGQMLLEVSYGGSPVPNPGIFFTYRENPVLRAFEPLRSFASGGRSINVTGQGFSLIQRFAMVVIAEPLQSWQPPREAESLQPMTVVGTDYVFHNDTKVVFLSPAVPEEPEAYNLTVLIEMDGHRALLRTEAGAFEYVPDPTFENFTGGVKKQVNKLIHARGTNLNKAMTLQEAEAFVGAERCTMKTLTETDLYCEPPEVQPPPKR

150-180 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.