PLP phosphatase Protein, Mouse (P.pastoris, His)
The PLP phosphatase protein plays a dual role as a pyridoxal phosphate (PLP) phosphatase and a protein serine phosphatase. Its enzymatic activity in vitamin B6 metabolism involves the dephosphorylation of PLP, PNP and PMP. PLP phosphatase Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived PLP phosphatase protein, expressed by P. pastoris , with N-His labeled tag.
- Species: Mouse
- Source: P. pastoris
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The PLP phosphatase protein plays a dual role as a pyridoxal phosphate (PLP) phosphatase and a protein serine phosphatase. Its enzymatic activity in vitamin B6 metabolism involves the dephosphorylation of PLP, PNP and PMP. PLP phosphatase Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived PLP phosphatase protein, expressed by P. pastoris , with N-His labeled tag.
Background
PLP phosphatase protein serves a dual function by acting as a pyridoxal phosphate (PLP) phosphatase, catalyzing the dephosphorylation of pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP) with a substrate preference order of PLP > PNP > PMP. This dual enzymatic activity positions it as a key player in vitamin B6 metabolism. Additionally, PLP phosphatase functions as a protein serine phosphatase, specifically targeting 'Ser-3' in proteins of the actin-depolymerizing factor (ADF)/cofilin family, including CFL1 and DSTN. This regulation of cofilin-dependent actin cytoskeleton reorganization is crucial for normal progression through mitosis and cytokinesis. Notably, PLP phosphatase does not dephosphorylate phosphothreonines in LIMK1, and it does not act on peptides containing phosphotyrosine. The multifaceted role of PLP phosphatase highlights its importance in both vitamin B6 metabolism and the dynamic regulation of the actin cytoskeleton, contributing to essential cellular processes.
Technical Parameters
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Species Mouse
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Source P. pastoris
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Tag N-His
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Accession
P60487 (M1-D292)
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Molecular Construction
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N-term
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His
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PLP phosphatase (M1-D292)
Accession # P60487 -
C-term
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Protein Length
Full Length
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Synonyms
PDXP; Pyridoxal (Pyridoxine, Vitamin B6) Phosphatase; Pyridoxal Phosphatase; PLP Phosphatase; Chronophin; FLJ32703; CIN; PLP; Pyridoxal Phosphate Phosphatase; Testicular Secretory Protein Li 36; DJ37E16.5; PLPP
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AA Sequence
MARCERLRGAALRDVLGQAQGVLFDCDGVLWNGERIVPGAPELLQRLARAGKNTLFVSNNSRRARPELALRFARLGFAGLRAEQLFSSALCAARLLRQRLSGPPDASGAVFVLGGEGLRAELRAAGLRLAGDPGEDPRVRAVLVGYDEQFSFSRLTEACAHLRDPDCLLVATDRDPWHPLSDGSRTPGTGSLAAAVETASGRQALVVGKPSPYMFQCITEDFSVDPARTLMVGDRLETDILFGHRCGMTTVLTLTGVSSLEEAQAYLTAGQRDLVPHYYVESIADLMEGLED
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Predicted Molecular Mass
33.5 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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