PNKP Protein, Human (His-SUMO)
Based on 1 Customer Validation
PNKP protein plays a key role in DNA damage repair through NHEJ and BER pathways. Its dual catalytic activity includes removal of the 3'-phosphate group from the DNA backbone and phosphorylation of the 5'-hydroxyl group, ensuring compatibility of DNA ends during extension and ligation. PNKP Protein, Human (His-SUMO) is the recombinant human-derived PNKP protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
PNKP protein plays a key role in DNA damage repair through NHEJ and BER pathways. Its dual catalytic activity includes removal of the 3'-phosphate group from the DNA backbone and phosphorylation of the 5'-hydroxyl group, ensuring compatibility of DNA ends during extension and ligation. PNKP Protein, Human (His-SUMO) is the recombinant human-derived PNKP protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
Background
PNKP Protein assumes a crucial role in the repair of DNA damage, actively participating in both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Employing its two catalytic activities, PNKP plays a pivotal role in maintaining DNA termini compatibility for subsequent extension and ligation processes. It accomplishes this by either removing 3'-phosphates from the DNA backbone or phosphorylating 5'-hydroxyl groups on the ribose sugar. This dual functionality highlights PNKP's significance in resolving various types of DNA lesions, ensuring the integrity of the genome and contributing to the intricate network of cellular mechanisms dedicated to DNA repair and maintenance.
Verified Bioactivity
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-His;N-SUMO
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Accession
Q96T60-1 (M1-G521)
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Molecular Construction
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N-term
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6*His-SUMO
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PNKP (M1-G521)
Accession # Q96T60-1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
PNKP; Homo Sapiens Polynucleotide Kinase 3'-Phosphatase (PNKP); Polynucleotide Kinase 3'-Phosphatase; EIEE10; Bifunctional Polynucleotide Phosphatase/Kinase; CMT2B2; DNA 5'-Kinase/3'-Phosphatase; MCSZ; PNK; AOA4; Polynucleotide Kinase-3'-Phosphatase
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AA Sequence
MGEVEAPGRLWLESPPGGAPPIFLPSDGQALVLGRGPLTQVTDRKCSRTQVELVADPETRTVAVKQLGVNPSTTGTQELKPGLEGSLGVGDTLYLVNGLHPLTLRWEETRTPESQPDTPPGTPLVSQDEKRDAELPKKRMRKSNPGWENLEKLLVFTAAGVKPQGKVAGFDLDGTLITTRSGKVFPTGPSDWRILYPEIPRKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVEAVVEKLGVPFQVLVATHAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPGRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSGPLCLPESRALLSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLGSWQRCVTTCETALKQGKRVAIDNTNPDAASRARYVQCARAAGVPCRCFLFTATLEQARHNNRFREMTDSSHIPVSDMVMYGYRKQFEAPTLAEGFSAILEIPFRLWVEPRLGRLYCQFSEG
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Predicted Molecular Mass
73.1 kDa
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Molecular Weight
Approximately 70 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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