PNKP Protein, Human (His-SUMO)

PNKP protein plays a key role in DNA damage repair through NHEJ and BER pathways. Its dual catalytic activity includes removal of the 3'-phosphate group from the DNA backbone and phosphorylation of the 5'-hydroxyl group, ensuring compatibility of DNA ends during extension and ligation. PNKP Protein, Human (His-SUMO) is the recombinant human-derived PNKP protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of PNKP Protein, Human (His-SUMO) is 521 a.a., with molecular weight of ~70 kDa.

PNKP Protein assumes a crucial role in the repair of DNA damage, actively participating in both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Employing its two catalytic activities, PNKP plays a pivotal role in maintaining DNA termini compatibility for subsequent extension and ligation processes. It accomplishes this by either removing 3'-phosphates from the DNA backbone or phosphorylating 5'-hydroxyl groups on the ribose sugar. This dual functionality highlights PNKP's significance in resolving various types of DNA lesions, ensuring the integrity of the genome and contributing to the intricate network of cellular mechanisms dedicated to DNA repair and maintenance.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-His;N-SUMO

Q96T60 (1M-521G)

11284  [NCBI]

MGEVEAPGRLWLESPPGGAPPIFLPSDGQALVLGRGPLTQVTDRKCSRTQVELVADPETRTVAVKQLGVNPSTTGTQELKPGLEGSLGVGDTLYLVNGLHPLTLRWEETRTPESQPDTPPGTPLVSQDEKRDAELPKKRMRKSNPGWENLEKLLVFTAAGVKPQGKVAGFDLDGTLITTRSGKVFPTGPSDWRILYPEIPRKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVEAVVEKLGVPFQVLVATHAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPGRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSGPLCLPESRALLSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLGSWQRCVTTCETALKQGKRVAIDNTNPDAASRARYVQCARAAGVPCRCFLFTATLEQARHNNRFREMTDSSHIPVSDMVMYGYRKQFEAPTLAEGFSAILEIPFRLWVEPRLGRLYCQFSEG

Approximately 70 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.