PPT1 Protein, Mouse (HEK293, His)

PPT1 Protein, an enzyme in lysosomal degradation, crucially removes thioester-linked fatty acyl groups, like palmitate, from modified cysteine residues in proteins or peptides. It prefers acyl chain lengths from 14 to 18 carbons. PPT1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived PPT1 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of PPT1 Protein, Mouse (HEK293, His) is 279 a.a., with molecular weight of 33 kDa.

PPT1 Protein, an enzyme involved in lysosomal degradation, plays a crucial role in removing thioester-linked fatty acyl groups, such as palmitate, from modified cysteine residues in proteins or peptides. It exhibits a preference for acyl chain lengths ranging from 14 to 18 carbons.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

HEK293

C-10*His

O88531 (D28-K306)

19063  [NCBI]

DPPSPPPLVIWHGMGDSCCNPMSMGVIKKMVEKEIPGIYVLSLEIGKNMMEDVENSFFLNVNVQVNMVCQILEKDPKLQQGYNAIGFSQGGQFLRAVAQRCPTPPMMTLISVGGQHQGVFGLPRCPGESSHICDFIRKSLNAGAYSKLVQERLVQAQYWHDPIKESVYRNYSIFLADINQERCVNESYKKNLMALKKFVMVKFFNDSIVDPVDSEWFGFYRSGQAKETIPLQESTLYTEDRLGLKKMDKAGKLVFLAKEGDHLQISKEWFTAHIIPFLK

33 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.