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PPT1 Protein, Mouse (HEK293, His)

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Based on 1 publication(s) in Google Scholar

PPT1 Protein, an enzyme in lysosomal degradation, crucially removes thioester-linked fatty acyl groups, like palmitate, from modified cysteine residues in proteins or peptides.It prefers acyl chain lengths from 14 to 18 carbons.PPT1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived PPT1 protein, expressed by HEK293 , with C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

PPT1 Protein, an enzyme in lysosomal degradation, crucially removes thioester-linked fatty acyl groups, like palmitate, from modified cysteine residues in proteins or peptides.It prefers acyl chain lengths from 14 to 18 carbons.PPT1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived PPT1 protein, expressed by HEK293 , with C-10*His labeled tag.

Background

PPT1 Protein, an enzyme involved in lysosomal degradation, plays a crucial role in removing thioester-linked fatty acyl groups, such as palmitate, from modified cysteine residues in proteins or peptides. It exhibits a preference for acyl chain lengths ranging from 14 to 18 carbons.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-10*His
  • Accession

    O88531 (D28-K306)

  • Gene ID
    19063  [NCBI]
  • Molecular Construction
    • N-term
    • PPT1 (D28-K306)
      Accession # O88531
    • 10*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    PPT1; INCL; Prev. PPT; Ceroid-Lipofuscinosis, Neuronal 1, Infantile; CLN1; PPT-1; Palmitoyl-Protein Hydrolase 1; Palmitoyl-Protein Thioesterase 1; Ceroid-Palmitoyl-Palmitoyl-Protein Thioesterase 1

  • AA Sequence

    DPPSPPPLVIWHGMGDSCCNPMSMGVIKKMVEKEIPGIYVLSLEIGKNMMEDVENSFFLNVNVQVNMVCQILEKDPKLQQGYNAIGFSQGGQFLRAVAQRCPTPPMMTLISVGGQHQGVFGLPRCPGESSHICDFIRKSLNAGAYSKLVQERLVQAQYWHDPIKESVYRNYSIFLADINQERCVNESYKKNLMALKKFVMVKFFNDSIVDPVDSEWFGFYRSGQAKETIPLQESTLYTEDRLGLKKMDKAGKLVFLAKEGDHLQISKEWFTAHIIPFLK

  • Predicted Molecular Mass

    33 kDa

  • Molecular Weight

    Approximately 38 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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