PTP4A1 Protein, Human (His)

The PTP4A1 protein is an active tyrosine phosphatase that not only stimulates the G1 to S phase progression of mitosis but also plays a crucial role in the development and maintenance of differentiated epithelial tissues. Its multifunctional contribution extends to cell differentiation, profoundly affecting cell behavior by enhancing proliferation, motility, and invasive activity. PTP4A1 Protein, Human (His) is the recombinant human-derived PTP4A1 protein, expressed by E. coli , with N-His labeled tag.

PTP4A1 protein, a dynamic protein tyrosine phosphatase, emerges as a key regulator that stimulates the progression from G1 into S phase during mitosis. Beyond its mitotic role, PTP4A1 is implicated in the development and maintenance of differentiating epithelial tissues, underscoring its versatile contributions to cellular differentiation. Notably, PTP4A1 exhibits a profound impact on cellular behavior, enhancing cell proliferation, motility, and invasive activity. In the context of cancer, PTP4A1 takes on a pivotal role, promoting metastasis. Its multifaceted functions highlight the crucial involvement of PTP4A1 in orchestrating diverse cellular processes, with implications for both normal tissue development and pathological conditions associated with cancer progression and metastasis.

Measured by its ability to cleave a substrate. p-Nitrophenvl phosphate (pNPP). The specific activity is > 0.5 pmol/min/μg.

Human

E. coli

N-8*His

Q93096 (M1-C170)

7803  [NCBI]

Partial

MARMNRPAPVEVTYKNMRFLITHNPTNATLNKFIEELKKYGVTTIVRVCEATYDTTLVEKEGIHVLDWPFDDGAPPSNQIVDDWLSLVKIKFREEPGCCIAVHCVAGLGRAPVLVALALIEGGMKYEDAVQFIRQKRRGAFNSKQLLYLEKYRPKMRLRFKDSNGHRNNC

Approximately 20.6 kDa,based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22μm filtered solution of 20mM Tris, 250mM NaCl, 1mM DTT, 20% Glycerol, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.