ROBO2 Protein, Human (HEK293, His)
Based on 1 Customer Validation
ROBO2 Protein, a receptor for SLIT2 and likely SLIT1, acts as a molecular guidance cue in cellular migration, especially in axonal navigation and axonal projection during neuronal development. Its interaction with SLIT2 underscores its crucial role in mediating responses to these molecular cues, indicating its essential involvement in guiding cellular movements and axonal patterning. ROBO2 Protein, Human (HEK293, His) is the recombinant human-derived ROBO2 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
ROBO2 Protein, a receptor for SLIT2 and likely SLIT1, acts as a molecular guidance cue in cellular migration, especially in axonal navigation and axonal projection during neuronal development. Its interaction with SLIT2 underscores its crucial role in mediating responses to these molecular cues, indicating its essential involvement in guiding cellular movements and axonal patterning. ROBO2 Protein, Human (HEK293, His) is the recombinant human-derived ROBO2 protein, expressed by HEK293 , with C-His labeled tag.
Background
The ROBO2 Protein functions as a receptor for SLIT2, and likely SLIT1, serving as a molecular guidance cue in cellular migration, particularly in axonal navigation at the ventral midline of the neural tube and the projection of axons to various regions during neuronal development. Its interaction with SLIT2 underscores its role in mediating responses to these molecular cues, suggesting a crucial involvement in guiding cellular movements and axonal patterning.
Verified Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Mouse Slit-3 at 1 μg/mL (100 μL/well) can bind biotinylated Human ROBO2. The Kd for this effect is 8.983 nM.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
Q9HCK4-1 (S22-P859)
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Molecular Construction
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N-term
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ROBO2 (S22-P859)
Accession # Q9HCK4-1 -
His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
ROBO2; Roundabout (Axon Guidance Receptor, Drosophila) Homolog 2; Roundabout Guidance Receptor 2; Roundabout Axon Guidance Receptor-Like Protein 2; KIAA1568; Roundabout, Axon Guidance Receptor, Homolog 2; Roundabout Homolog 2; SAX3; Roundabout, Axon Guida
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AA Sequence
SRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQP
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Molecular Weight
Approximately 116 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (240 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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