ROCK2 Protein, Human (Active, 550a.a, sf9, His-GST)
ROCK2 Protein, Human (Active, 550a.a, sf9, His-GST) is the recombinant human-derived ROCK2, expressed by Sf9 insect cells , with N-6*His and N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
ROCK2 Protein, Human (Active, 550a.a, sf9, His-GST) is the recombinant human-derived ROCK2, expressed by Sf9 insect cells , with N-6*His and N-GST labeled tag.
Background
ROCK2 is a protein kinase that serves as a key regulator of the actin cytoskeleton and cell polarity. It modulates smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion, and motility by phosphorylating proteins such as ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A, and VIM. Additionally, ROCK2 phosphorylates SORL1 and IRF4 and acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. During myogenic differentiation, it positively regulates the activation of p42/MAPK1-p44/MAPK3 and p90RSK/RPS6KA1. ROCK2 plays a crucial role in the timely initiation of centrosome duplication and inhibits keratinocyte terminal differentiation. It may regulate eyelid closure and ventral body wall formation by organizing actomyosin bundles. In the hippocampus, ROCK2 is critical for regulating spine and synaptic properties and contributes to the circadian rhythm of aortic myofilament Ca2+ sensitivity and vascular contractility by modulating myosin light chain phosphorylation.
Technical Parameters
-
Species Human
-
Source Sf9 insect cells
-
Tag N-6*His;N-GST
-
Accession
O75116 (P5-T554)
-
Gene ID9475
-
Molecular Construction
-
N-term
-
6*His-GST
-
ROCK2 (P5-T554)
Accession # O75116 -
C-term
-
-
Protein Length
Partial
-
Synonyms
ROCK2; ROCK-II; Rho Associated Coiled-Coil Containing Protein Kinase 2; Non-Specific Serine/Threonine Protein Kinase; Rho-Associated, Coiled-Coil-Containing Protein Kinase II; Rho-Associated Protein Kinase; Rho-Associated Protein Kinase 2; Rho Kinase 2; P
-
AA Sequence
PPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQIST
-
Predicted Molecular Mass
93.2 kDa
-
Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
